echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > 1× method for the preparation of TATE buffer.

    1× method for the preparation of TATE buffer.

    • Last Update: 2020-10-19
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    generally pre-provisioned 50 times the TATE buffer, diluted to 1x in time. 50x TATE buffer is made by 1. It is stated that the following in 1L " berred: Tris242g Na2 edta.2H2 O37.2g 2. Add about 600mL of deionized water to the beech and stir to dissolve. 3. Add 57.1mL of acetic acid and stir well. 4. Add deionized water to hold the solution to 1L and store it at room temperature. 50XTAE dosing: pre-provisioned 0.5M EDTA pH8.0 buffer (in molecular cloning terms, suspended with EDTA dodium salt) Add a moderate amount of sodium hydroxide to the solution, stirring, until the solution is clarified, at this time the solution pH is about 8); and then the appropriate amount of TRIS dissolved in the appropriate amount of water, added EDTA storage liquid and ice acetic acid, fixed mix can be, according to the size of the experimental use can be matched with 1-10L. See the answer above for a specific recipe. 1XTAE (1L) 50XTAE 20ml H2Oto 1L Note: EDTA should be based on the number of acid ion moles to change the quality of the drug, because its related products have sodium without water, there is two sodium two water, should be carefully considered.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.