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Hello teachers! The new phase of 10× single-cell transcriptome common Q&A is here again! In the previous issue, we introduced some of the biggest questions that need to be understood before the single-cell experiment is carried out (click to read
).
In this issue, we will introduce the details
of quality control in the single-cell experiment.
1.
transcriptional bursting
Figure 1 Schematic diagram of the phenomenon of transcription bursts under the microscope
2.The overall flow of single-cell experiments
Figure 2 Overall flowchart of a single-cell experiment
2.
1 Cell isolation
Figure 3 Pay attention to problems when cell isolation
2.2 Cell sorting
Figure 4 Chromium X cell sorting
2.
3 Cell lysis 2.
4 Reverse transcription and PCR amplification
Figure 5 10× single-cell flow
2.
5 Library building and sequencing
Fig.
6 Single-cell library quality inspection peak diagram
6 Single-cell library quality inspection peak diagram
summary
