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In recent years, the development of spatial transcriptome technology has been in full swing.
Due to its broad application prospects, it has become a research hotspot in the field of life sciences
.
As a fast-growing company, 10x Genomics has been committed to researching and developing new technologies, launching new products and expanding product lines.
The Visium spatial gene expression solution it developed combines histology with spatial resolution whole transcriptome analysis.
Gene expression can be localized and quantified in a tissue context
.
FFPE samples are very common research materials in clinical practice.
Pathological samples can be stored for a long time.
Because they can be traced to clinical information such as disease diagnosis and prognosis, they have high clinical research value.
However, severe nucleic acid degradation and molecular cross-linking make them Be a precious but at the same time challenging sample type
.
In order to solve the problem of serious degradation of FFPE samples, 10x Genomics launched the first-generation product of Visium FFPE gene expression solution in 2021, which can analyze the whole transcriptome of long-term preserved FFPE tissue samples, and has complete tissue coverage, which can be reproduced Analyze long-term stored biobank samples for biomarker discovery, or perform retrospective and longitudinal studies
.
In order to unlock FFPE samples in more scenarios, such as archived samples pasted on ordinary glass slides, large-area tissue samples, and plans to initially screen the samples to determine the region of interest before conducting subsequent experiments, 10x Genomics recently Launched the first Visium instrument, CytAssist, a small benchtop instrument that bridges histology and genomics by transferring transcriptomic analytes from FFPE tissue on regular slides to 10x Visium gene expression slides
.
Due to its broad application prospects, it has become a research hotspot in the field of life sciences
.
As a fast-growing company, 10x Genomics has been committed to researching and developing new technologies, launching new products and expanding product lines.
The Visium spatial gene expression solution it developed combines histology with spatial resolution whole transcriptome analysis.
Gene expression can be localized and quantified in a tissue context
.
FFPE samples are very common research materials in clinical practice.
Pathological samples can be stored for a long time.
Because they can be traced to clinical information such as disease diagnosis and prognosis, they have high clinical research value.
However, severe nucleic acid degradation and molecular cross-linking make them Be a precious but at the same time challenging sample type
.
In order to solve the problem of serious degradation of FFPE samples, 10x Genomics launched the first-generation product of Visium FFPE gene expression solution in 2021, which can analyze the whole transcriptome of long-term preserved FFPE tissue samples, and has complete tissue coverage, which can be reproduced Analyze long-term stored biobank samples for biomarker discovery, or perform retrospective and longitudinal studies
.
In order to unlock FFPE samples in more scenarios, such as archived samples pasted on ordinary glass slides, large-area tissue samples, and plans to initially screen the samples to determine the region of interest before conducting subsequent experiments, 10x Genomics recently Launched the first Visium instrument, CytAssist, a small benchtop instrument that bridges histology and genomics by transferring transcriptomic analytes from FFPE tissue on regular slides to 10x Visium gene expression slides
.
10x Genomics provides complete solutions from instrumentation, reagent consumables to analysis software
.
This assisted instrument is compatible with FFPE samples stained by H&E or immunofluorescence (IF).
The open source user-friendly software Space Ranger and Loupe Browser can perform downstream data processing analysis and visual display
.
.
This assisted instrument is compatible with FFPE samples stained by H&E or immunofluorescence (IF).
The open source user-friendly software Space Ranger and Loupe Browser can perform downstream data processing analysis and visual display
.
After deparaffinization and imaging of FFPE samples placed on common standard slides, de-crosslinking and probe hybridization, two standard slides and a Visium slide with two capture zones were placed in In the CytAssist instrument, the tissue section on the standard slide is aligned with the top of the two capture areas on the Visium slide
.
Instrument captures brightfield images to provide spatial localization for calibration and data analysis, then hybridizes transcriptome analytes to Visium slides, enabling transfer of tissue transcriptome analytes from plain to Visium slides, followed by probe extension Operations such as library construction are the same as the Visium FFPE standard process, which can be completed outside the instrument
.
.
Instrument captures brightfield images to provide spatial localization for calibration and data analysis, then hybridizes transcriptome analytes to Visium slides, enabling transfer of tissue transcriptome analytes from plain to Visium slides, followed by probe extension Operations such as library construction are the same as the Visium FFPE standard process, which can be completed outside the instrument
.
10x Genomics has also upgraded the original Visium FFPE product to v2.
0, which includes newly designed slides and reagents that are compatible with the Visium CytAssist instrument
.
0, which includes newly designed slides and reagents that are compatible with the Visium CytAssist instrument
.
The upgraded Visium FFPE v2.
0 product has the following features:
① The upgraded glass slides have higher adhesion to tissues, and formal experiments can be carried out without pre-testing
About 90% of the genes have three pairs of probes designed, and one pair of probes has been designed for some highly expressed genes, with high sensitivity and high specificity
③ Provide commercial 35 protein combination kits, which can be used for the same FFPE sample Simultaneous analysis of transcriptome and protein, and simultaneous detection of dozens of proteins for gene expression detection
④ Expanded detection range: the quality inspection standard is DV200>30%, and more samples with high degradation degree can be detected
⑤ Configuration upgrade: newly added A large 11mm*11mm capture area unlocks more possibilities
.
0 product has the following features:
① The upgraded glass slides have higher adhesion to tissues, and formal experiments can be carried out without pre-testing
About 90% of the genes have three pairs of probes designed, and one pair of probes has been designed for some highly expressed genes, with high sensitivity and high specificity
③ Provide commercial 35 protein combination kits, which can be used for the same FFPE sample Simultaneous analysis of transcriptome and protein, and simultaneous detection of dozens of proteins for gene expression detection
④ Expanded detection range: the quality inspection standard is DV200>30%, and more samples with high degradation degree can be detected
⑤ Configuration upgrade: newly added A large 11mm*11mm capture area unlocks more possibilities
.
Both Visium CytAssist instruments and FFPE v2.
0 products are now available for order, order early and ship early! Welcome to contact us in the following ways, Tel: 010-84409661, Email: marketing@emtd.
com.
cn
.
0 products are now available for order, order early and ship early! Welcome to contact us in the following ways, Tel: 010-84409661, Email: marketing@emtd.
com.
cn
.
