20S protease: PBE1 gene expression is also induced by salt stress.

The 26S protease system regulates plant growth and response to environmental stress by effectively degrading many key protein factors.
26 protease system consists of two subcomplexes: 20S protease and 19S protease.
20S protease are assembled into a hollow cylinder structure by multiple alpha subakis and beta subakis in the form of alpha 1-7/beta1-7/beta-1-7/alpha1-7.
its sub-based mutations are closely related to the emergence of many diseases in humans, including cardiovascular disease, diabetes, neurological diseases and cancer.
interesting, in addition to standard proteases in the human body, 20S proteases also have three tissue-specific forms/types: immunotype protease, thymus protease and sperm-type protease.
, oxidative stress can also induce the production of coercent protease.
these specific forms of protease mediate the degradation of specific proteins in specific cellular environments.
However, there have been no reports of specific proteases that depend on specific types or environments of cells in plants.
, Xie Qi, a researcher at the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, systematically compared the sub-cells of 20S protease purified in the pontoon seedlings of the adhemanal mustard seedlings with salt stress and uncoercive treatment, and found that salt stress increased the abundance of beta 5 sub-pBE protein in 20S proteases.
at the same time, it was found that PBE1 gene expression was also induced by salt stress. the lack of function of
PBE1 impairs the normal assembly of the amoeba 26S protease body under salt stress and reduces the activity of the protease body.
these experimental results reveal that there is also a specific coermatic protease in plants. Further research
found that the absence of PBE1 caused the degradation of ABI5, the key transcription factor of the ABA signal path, to inhibit the degradation of the ABA signal path, which affected the expression of a series of response genes downstream of the ABA signal, resulting in the Aba sensitive phenotype of the PBE1 mutant leaf withunchanged green.
, therefore, the complete protease assembly of PBE1 medial is necessary for the successful establishment of the self-cultivation of amoeba.
, the study reveals that PBE1, by regulating the assembly and activity of proteases, forms a coercative-specific protease, participates in the response of plant abiotic stress signals, and regulates the conversion process of plant seedlings from heterotrophic growth to self-cultivation.
the findings were published online October 10 in the Journal of New Phytologist (DOI: 10.1111/nph.15471). Han Jiajia, a postdoctoral fellow who has been out of the
Xieqi Research Group, is the first author of the paper.
Xie Qi and Professor Liu Jianxiang of Zhejiang University are co-authors.
the research was funded by the Ministry of Science and Technology's major protein projects and the NSFC project.
Source: Institute of Genetics and Developmental Biology.