8-O-acetyl jasenoside methyl ester

8-O-acetyl jasenoside methyl ester

8-Acetylshanzhiside methyl ester

【Structural formula】

[Molecular formula and molecular weight] C ₁₉ H ₂₈ O ₁₂ ; 448.

【Source】Lamiophlomis rotata (Benth.

【Properties】White crystals

This product is easily soluble in methanol and ethanol

The melting point is 104～105℃

【Purity Check】

TLC

1.

Developing solvent chloroform - methanol (4:1)

Detectable element phosphomolybdic acid test solution was heated at 105 ℃ to spot color clear view under sunlight

2.

Developing solvent ethyl acetate - methanol (7:1)

Check the phosphomolybdic acid test solution, heat it at 105°C until the spots appear clear, and inspect under sunlight

High performance liquid chromatography

Chromatographic column Agilent SB C ₁₈ column, 5um (4.

Mobile phase acetonitrile - water (15:85), flow rate 1ml / min

Measurement wavelength 235nm

Differential calorimetry

Start temperature 50℃, end temperature 300℃, heating rate 5℃/min

【Structural identification】235

3420, 1710, 1635, 1375, 1280, 1080, 860

FAB-MS m/z: 471[M+Na] ⁺ , 487[M+K] ⁺

¹ H-NMR (CD ₃ OD) δ: 1.

¹³ C-NMR (CD ₃ OD) δ: 96.

【Storage】Keep refrigerated