A map of gene expression that is specific to different areas of the top of the stem and leaves.

Gene differential expression is the basis of cell differentiation and specific functions in different cell types. Transcription maps of
cell characteristics are critical to understanding how different types of cells grow and develop and respond to the environment.
but plant cells are fixed by cell walls, not easy to isolate, it is difficult to obtain the specific transcription data of cell type.
the Research Group of the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences has established a cell-specific expression map of the organ boundary region (Tian et al., 2014, Molecular Systems Biology) in previous work.
on this basis, the team used translation ribosome affinity and purification techniques to further obtain specific gene expression maps of the top of the stem and different regions of the leaves.
study found many region-specific unknown cell functions, and found that cell types with low similarity can use common regulatory modules.
the study has further identified new regulatory genes for the initial regulation of lateral biological tissues.
map provides important reference data for the study of plant growth and development and environmental response.
a database built through the study, the cell-specific expression data of each gene can be retrieved and visualized.
diagram: (a) a diagram of cell types in different areas of the stem and blades.
(b) analysis of the main components of translation groups of different regional types.
(c)eFP browser shows the expression patterns of the HAN gene in different areas at the top of the stem.
(d) the co-expression module significantly enriches the genes associated with the initiation of the side-by-side biogenesis.
(e) newly identified phenotype missing from the mutant side buds of HAN, a gene associated with the initiation of lateral biogenes.
the findings were published in the journal Nature Communications on January 11, 2019.
,
, The Deputy Researcher of the Jiaoyu Bell Research Group, Tian Caihuan, is the first author of this paper, and the CooYu Bell is the communication author.
Associate Professor Wang Ying of the University of Chinese Academy of Sciences, Dr. Yu Haoxuan of Sichuan University, Professor Nicholas Provart of the University of Toronto and Professor Elliot Meyerowitz of the California Institute of Technology were also involved in the study.
the research was supported by the National Natural Science Foundation of China, Newton Senior Scholars, THE Special Gm Special and the Chinese Academy of Sciences Youth Innovation Promotion Association.
Source: Institute of Genetics and Developmental Biology.