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"A method for extracting total RNA from tissues with high content of sandalwood essential oil" won the invention patent

 Last Update: 2021-12-30
 Source: Internet
 Author: User

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It was learned on December 10 that "a method for extracting total RNA from sandalwood essential oil tissues with high content of sandalwood" completed by Zhang Xinhua and other researchers from the South China Botanical Garden of the Chinese Academy of Sciences was authorized by the national invention patent

Take sandalwood wood tissue and grind it into powder with liquid nitrogen, add it to the Tris-HCl extract, freeze it at -80° , centrifuge to take the supernatant, add sodium acetate and isopropanol to precipitate RNA , and dissolve the precipitate with TE buffer after centrifugation , Then add cetyltrimethylammonium bromide and mix well, extract with chloroform / isoamyl alcohol, centrifuge, take the supernatant, add lithium chloride overnight to precipitate RNA , centrifuge, and wash with ethanol to obtain total RNA

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