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We propose a sensitive and accurate analytical method for quantifying 29 current-use pesticides in human serum or plasma. These pesticides include organophosphates, carbamates, chloroacetanilides, and synthetic pyrethroids, pesticides used in both agricultural and residential settings. Our method employs simple solid-phase extraction followed by highly selective analysis using isotope dilution gas chromatography-high-resolution mass spectrometry. The method is very accurate, has limits of detection (LODs) in the low picogram/gram range, and has coefficients of variation that are typically less than 20% at the low picogram/gram end of the method linear range.