“A Na+/K+/2Cl-cotransporter NKCC gene and its application in Penaeus monodon Na+/K+/2Cl-cotransporter” from the South China Sea Institute of Chinese Academy of Fishery Sciences was granted the national invention patent authorization

Recently, the China foodmate.
net/tag_1737.
html" class="zdbq" title="Aquatic related food information" target="_blank">Fisheries Sea Fisheries Research Institute Academy of Sciences ZHOU Falin, Liyun Dong, JIANG Shigui inventors to "a spot section foodmate.
net/tag_409.
html" class="zdbq" title="Prawn related food information" target="_blank">prawn Na / K + / 2Cl- + cotransporter foodmate.
net/tag_3855.
html" class="zdbq" title="Protein related food information" target="_blank">protein NKCC foodmate.
net/tag_847.
html" class="zdbq" title="Gene-related food information" target="_blank">gene and its applications" national invention patents, patent No.
ZL2019910862668.
X.
The invention has the advantages of using RACE technology to obtain the full length of the cDNA sequence of Penaeus monodon NKCC, analyzing its expression patterns in different tissues of the monodon and during foodmate.
net/tag_321.
html" class="zdbq" title="Low-salt related food information" target="_blank">low-salt stress, and can provide a basis for analyzing the regulatory mechanism of the response of Penaeus monodon to low-salt stress Data and other advantages.

 

　　The invention patent passed PlInk performed an association analysis between these two loci, and the results showed that the T gene frequencies of PmNKCC2061 and PmNKCC 2157 were significantly lower in the low-salt-tolerant extreme population than in the low-salt-tolerant extreme population ( P <0.
05).
This shows that the T mutations at PmNKCC2061 and PmNKCC 2157 are negatively correlated with the low-salt tolerance of P.
monodon, and populations with weak low-salt tolerance tend to have higher frequency of these two genes.
It is preliminarily believed that the PmNKCC2061 locus and PmNKCC 2157 locus are associated with the low salt tolerance traits of P.
monodon.
PmNKCC2061 locus and PmNKCC 2157 locus can be used as candidate sites for further verification, in order to be used in the future low salt tolerance of P.
monodon In the process of breeding new strains.