A new adenine base editor minimizes cell RNA editing

a new study, researchers from the Broad Institute and Harvard University in the United States have found evidence that using a base editor can lead to unexpected RNA editing in cells. The findings, published May 8, 2019 in the journal Science Advances, are titled "Analysis and analysis of cellular RNA editing by DNA adenine base editors." In this paper, they describe their study of the CRISPR type adenine base editor (ABE) and their findings.ABE converts one DNA base pair into another, allowing for the repair of mutations in certain cell types without unwanted editing effects. Abe is also thought to have the potential to correct almost half of the known genetic abnormalities that cause medical diseases. The scientific basis of ABE is becoming more and more important to the medical community. Unfortunately, some recent studies have found that ABE can also lead to unexpected editing. In March, a team of researchers found that Cytosine Base Editor Type 3 (CBE3) induces single nucleotide variation at a higher-than-normal rate. Last month, another team of researchers found that cytosine base editors (BBEs) and ABEs cause off-target editing in RNA. In the new study, the researchers tried to further test off-target editing when using ABE and find a solution after confirmation.the researchers analyzed the latest version of ABE, called ABEmax, in a way that included transcripts of all cells in the human cell line, and they used more sensitive tools than others to do so. They reported that they did find low levels of off-target editing in RNA samples. To solve this problem, they developed a new ABE variant (based on inactivated wild E. coli) that retains the ability to edit at the target base while leading to less RNA editing. They further reported that the new variants were built in a way that decoupled the RNA and DNA editing processes, minimizing off-target editing in DNA and RNA.the researchers concluded that because of their lower RNA editing and shorter RNA half-life, the degree of interference with future research may depend on their specific application. They suggest that scientists seeking to minimize RNA editing use a new variant of the --- they call ABEmaxQW. (Bio Valley Bioon.com)