A new breakthrough has been made in the ultra-trace biological sensing technology of single crystal graphene.

Biotechnology Channel News: Recently, the research team led by Dr. Xu Shicai, Professor Wang Jihua and Professor Zhou Yaoqi of the Provincial Key Laboratory of Biophysics, Texas College, published a study entitled "Real-time reliable reselydmark of the binding kinetics of DNA hybrid" in the March 21 issue of the journal Nature Communications. A new method for detecting molecular interaction dynamics and affinity has been reported in a research paper on the multi-channel graphene biosensor, a multichannel graphene biosensor for real-time and reliable detection of DNA hybrid dynamics and affinity. The dynamics and affinity analysis of
molecular interactions have always been an important part of the field of life science, which not only involves the analysis of key processes in biology such as gene expression, DNA replication, signal conduction, cell-to-cell interaction, but also is the basis of important analytical techniques such as immunoanalysis, biosensors and DNA hybridization, and has broad application prospects in the fields of scientific research, disease diagnosis, environmental monitoring, biotechnology, drug development and food safety testing.
For a long time, surface plasma resonance (SPR) is the most commonly used method to detect molecular interaction dynamics and affinity, which has the advantages of not requiring sample marking and low sample dosing, but also has the disadvantages of high analytical cost, low analysis volume and insufficient accuracy in measuring small molecules.
, it is urgent to develop a new method of dynamic and affinity analysis.
graphene biosensing technology with graphene as a sensitive material, can achieve small molecule sensitivity detection, and with large-scale integrated circuit compatibility, easy miniaturization, low cost and easy to achieve high-volume detection and other outstanding advantages, it is very promising to develop into a new generation of nano-biosensitive analysis and detection technology.
However, due to the defects of the crystal structure of conventional graphene itself and the damage of molecular probe conceding to the structure of graphene's character, graphene biorecessing technology is difficult to use for dynamic and affinity testing.
In recent years, Dr. Xu Shicai, Professor Wang Jihua and Professor Zhou Yaoqi have led a research team that has conducted in-depth research around monocrystalline graphene ultra-trace biosensor technology, made significant progress in the use of graphene biosensors for dynamics and affinity, and creatively adopted centimeter-grade monocrystalline Graphene, as a conductive channel, has developed a single crystal graphene ultra-trace biosensor, and cleverly used the non-co-priced even probe to avoid the damage of probe molecules to the graphene-based structure, thus promoting reliable graphene biosensor sensing technology has taken a key step.
On this basis, for the first time, multi-channel single-crystal graphene ultra-trace biosensors were used to present molecular interactions directly with electrical signal changes, and the real-time detection of DNA hybrid dynamics process was successfully realized, with a low limit of 10 pM, 3 orders of magnitude lower than conventional SPR;
This study provides a new dynamic and affinity analysis detection method with single crystal graphene ultra-trace biosensors as the tool, solves the problem that SPR method is difficult to detect small molecules, integrates the lower concentration interaction system into the dynamic analysis range, and has the characteristics of high acne, thus greatly expanding the research object of dynamics and affinity, which is of great significance for discovering the new law of interaction in biology and promoting the application of dynamic analysis detection method in a wider field.
work was carried out in collaboration with researchers at Griffith University and Shandong Normal University, based on the provincial key laboratory for biophysics at Texas College.
this work has been supported by the National Natural Science Foundation of China (11604040, 61671107, 11674199 and 11547225), the Shandong Natural Science Foundation Project (ZR2014FQ032) and the Taishan Scholars Project.
Texas College as an undergraduate college, this is the first paper published in nature newsletter to name Texas College as the first communications unit.
Schools have always attached great importance to scientific research work, adhere to the development path of connotation, in recent years, in SCI three or more journals published 26 papers, CSSCI journals published 10 papers, published 7 academic works, approved the National Natural Science Foundation of China 7 projects, the National Social Science Foundation late-stage funding projects 1, 47 provincial and municipal scientific research projects, approved vertical scientific research funds of 5 million yuan.
, a sub-issue of Nature, was first published in April 2010 and published online by the Nature Publishing Group in the UK, with an impact factor of 11.329 in 2015.
The journal publishes high-quality research results with important breakthroughs in the field of natural sciences, and its academic content, in addition to biology, chemistry and physics, is devoted to publishing research results in a number of disciplines not covered by the Nature series, such as developmental biology, plant science, microbiology, ecology, palaeontology, astronomy, etc., and particularly welcomes the achievements of interdisciplinary research fields such as biophysics, bioengineering, chemical physics, environmental science, etc.
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