Plant distant hybridization is a method of hybrid breeding, which refers to the hybridization between different species, inter-genus plants and even more distantly related species, and has played a huge role in the cultivation of new crop varieties
However, compared with inbreeding, the genetic background of distant hybrids is more different, and it is more prone to hybridization obstacles and failure of hybridization
Recently, the team of Professor Teng Nianjun of Nanjing Agricultural University analyzed the molecular mechanism of plant embryonic development positive regulation transcription factor CmLEC1 and negative regulation transcription factor CmERF12 in chrysanthemum distant hybridization embryo abortion, and invented a new kind of overcoming plant distant relationship Methods of hybrid reproduction disorders
Horticulture Research Journal of Experimental Botany A positive regulatory transcription factor
A positive regulatory transcription factor In view of the widespread reproductive obstacles in distant hybridization of plants, Teng Nianjun’s team used chrysanthemum as the material in the previous research and found that embryo abortion was the main form of reproductive obstacles in distant hybridization
Teng Nianjun team Xu Sujuan , the first author of the paper, introduced that the paper published in "Research in Horticulture" provides a detailed analysis of the function and mechanism of CmLEC1
Xu Sujuan, the first author of the thesis, "Horticultural Research" The team found that overexpression of CmLEC1 transgenic chrysanthemum can promote the normal development of hybrid embryos and significantly increase the seed setting rate of distant hybridization, while the interference and down-regulation of amiR-CmLEC1 in transgenic chrysanthemums significantly reduces the seed setting rate of distant hybridization
In order to further study the molecular mechanism of CmLEC1 positively regulating the embryonic development of chrysanthemum, the team obtained the CmLEC1 interaction protein CmC3H through yeast library screening, and verified the interaction through experiments such as yeast two-hybrid
A negative regulatory transcription factor
A negative regulatory transcription factor The paper published in the "Journal of Experimental Botany" provides a detailed analysis of the function and mechanism of CmERF12
"Journal of Experimental Botany" According to Wu Ze, the co-first author of the paper, the team found that CmERF12 is specifically and highly expressed in aborted embryos obtained from chrysanthemum hybridization, and its protein is an AP2/ERF family transcription repressor containing an EAR motif
According to Wu Ze, the co-first author of the paper, Heterologous overexpression of CmERF12 in Arabidopsis will significantly reduce the Arabidopsis seed setting rate
In order to further study the molecular mechanism of CmERF12 negatively regulating embryonic development, the team obtained the CmERF12 interaction protein CmSUF4 through yeast library screening.
Further research found that CmSUF4 can directly bind to the promoter of the embryonic development-related gene CmEC1 and activate the expression of this gene
The birth of a new method
The birth of a new method The birth of a new method "The two papers analyzed the molecular mechanisms of CmLEC1 and CmERF12 regulating embryo development from both positive and negative aspects, and enriched the theoretical knowledge of plant transcription factors regulating embryo development
"Teng Nianjun said
Related paper information: https://doi.
https://doi.
org/10.
1093/jxb/erab398 https://doi.
org/10.
1093/jxb/erab398
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