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    Home > Food News > Food Articles > A new strategy for efficient and controllable positive culture of 3D cell balls

    A new strategy for efficient and controllable positive culture of 3D cell balls

    • Last Update: 2021-03-16
    • Source: Internet
    • Author: User
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    3D cell spheres, modeled on tumor micro-environment in vitro, are used to visually observe drug diffusion, immune escape, and deep therapy, and are ideal models for understanding the mechanisms of drug and cellular tissue action and evaluating the effectiveness of cancer treatment. At present, due to material and method limitations, in vitro 3D cell culture can not break through the size and stability of the constraints. This paper provides a new strategy for positive 3D size controlled cell sphere culture, which can be used in online observational studies of the mechanism of action between drugs and tumor tissue.
    current in vitro biological evaluation is mostly based on single-layer cell culture technology and developed, but in the real physiological environment, cells exist close and rich three-dimensional interconnecation. Therefore, the single-layer cell evaluation method can not accurately reflect the physiological activity of the cell's real micro-environment and its response behavior to external stimuli. Especially with the further study of tumor diseases, scientists have found that cell micro-environments have a crucial impact on drug resistance, immune escape, and drug diffusion. At present, in vitro tumor research methods can not provide three-dimensional structural characteristics in real environment, which will lead to the absence of physiological signals or even the wrong results. Therefore, the research hot spot of in vitro culture 3D sphere has been given birth.The establishment of
    three-dimensional cell sphere model has well filled the gap between in vitro and in vivo evaluation, which simulates the inherent metabolism (nutrients, oxygen, metabolites) and proliferation gradients in the natural physiological environment, and tracks the diffusion path of drugs, which is an ideal model for cell research. The emergence of this technology is of great significance to tumor treatment mechanism analysis, drug evaluation, stem cell differentiation and regenerative medicine. Existing methods for preparing 3D cell balls include the use of low-adhesive hydro-water surfaces, bioreacters based on rotational stirring, magnetic manipulation, and drip-suspension techniques. These methods have problems such as long culture cycle, shear stress destroying cell morphology, cell ball inverted can not be observed in place. Therefore, it is urgent to develop a new method of 3D cell ball construction that is efficient, safe and easy to observe in-place.
    this challenge, Professor Lu Qinghua's research team proposed a new method of constructing forward culture cell balls using ultra-double dredging surfaces. By building a highly performing fluorosilicon aerogel interface, they achieve a stable, ultra-double-clean surface. This surface can effectively block cell adhesion for a long time, while limiting the spread of cell fluid, in the interface pressure and gravity synergy, can be completed in a short period of time volume controlled cell ball preparation. The prepared 3D cell balls can be used for long-cycle online observation and research evaluation on the interface. The researchers proved that it has good application prospects in tissue fusion, drug evaluation and online tracking.
    it is worth mentioning that this is a positive culture technology, breaking through the traditional suspension of the size of the limits and inverted cell ball observation limitations. The method is also expected to produce three-dimensional spheres of complex cell line in vitro, reproducing the natural environment of cells. And its ultra-double-sparse interface features can be recycled. The cycle is simple and requires only the removal of the original culture, without any processing. The emergence of this method will expand the form of 3D cell ball evaluation, and provide new ideas and methods for simulating tumor micro-environment, tracking tumor development process and exploring tissue regeneration.
    the study was published in the National Science Review under the title "End Superamphibic Silica Aerogel Surfaces for the Culture of 3D Cellular Spheroids." Professor Lu Qinghua of Tongji University is the author of the paper, which was jointly conducted by Xu Lianyi, a teacher at Shanghai Institute of Electrical Engineering, and Chen Double, a postdoctoral student at Tongji University. (Source: Science Network)
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