A new theoretical framework for the exchange of symbiotic nutrients of the root of the main carbon source, the fungal fatty acids of the clumps.

On October 4, Molecular Plant published an online paper entitled Medicago AP2-Domain Researcher WRI5a Is a Master of Biosynthesis and Transfer Mycorrhzal Symbiosis of the Center for Excellence in Molecular Plant Sciences of the Chinese Academy of Sciences/Institute of Plant Physiology and Ecology of The SiTao.
the study found that the transcription factor WRI5a was a molecular switch for the exchange of fatty acid carbon sources and phosphorus nutrients during the symbiosis of the fungus root.
the vast majority of terrestrial plants, with the help of their own root system, through the formation of mutually beneficial symbiosis with the root fungus, efficiently obtain phosphorus, nitrogen and other nutrients from the environment, and pass the carbon source to the root fungus, to the ecosystem to enter carbon sources. The establishment of the symbiotic relationship between
and plants depends on signal communication and transduction between the root fungi and plants, Wang's research team has shown in recent years that OsCERK1 controls the identification of the root signal molecule, Plant Plant Factor (Plant Journal, 2015), and isolates the key transcription factor complex of root symbiosis IPD3-DellA-DIP1 (Cell, 2014; Nature Communications, 2016), and the HA1 gene that controls nutritional exchange downstream (The Plant Cell, 2014).
the latest research of the research team, the fungus root fungus induces plant synthesis of fatty acids, fatty acids through plant STR/STR2 transport protein into the root fungus, established a fatty acid as the main carbon source of nutrients of the root symbiotic nutrition exchange of a new theoretical framework (Science, 2017; Molecular Plant, 2017).
but the mechanism of transport regulation of carbon-based nutrients-fatty acids between plants and branch fungi is unclear.
the study showed that the transcription factor WRI5a was induced by Myc Factor secreted by the mycobacteria, which combined to activate the expression of the target gene in the AW-box region of the promoter.
experiments have shown that WRI5a can bind to AW-box on the STR promoter, thereby activating the expression of the fatty acid transporter STR and regulating the transport of fatty acids.
in the WRI5a gene mutants, the rate of infection of the root fungus decreased.
then the EXPRESSION of the WRI5a gene, the root fungus infection increased significantly, and the total fatty acids and C16:0 fatty acid content of plants were significantly increased, indicating that the WRI5a gene in the bacteria fungus infection process, the synthesis of plant fatty acids has a positive regulatory effect.
, the study also found that the WRI5a gene activates the expression of plant phosphorus transporter MtPT4 and has an important regulatory effect on plants absorbing phosphorus from the branch fungus.
postdoctoral student Jiang Yina and doctoral student Xie Qiuyu are the co-authors of this paper, the research is supported by the National Key Research and Development Program, the National Natural Fund Committee and the Chinese Academy of Sciences.
Source: Shanghai Institute of Life Sciences.