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A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLa Cells

 Last Update: 2020-12-18
 Source: Internet
 Author: User

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In cells, the poly(A) tail stimulates translation from messenger RNAs bearing a cap structure or viral
IR
ES elements. This3′ end-mediated stimulation of translation is not reflected in commonly used commercial cell-free translation systems preparedfrom rabbit reticulocytes or wheat germ. We describe a simple procedure to generate poly(A) tail-responsive translation extractsfrom HeLa cells. We suggest that this procedure should be adaptable to many animal cell lines.

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