A&R: Glutaminase 2 promotes CD4+ T cell production of interleukin 2 by supporting antioxidant defense in systemic lupus erythematosus

Objective : The glutaminase (GLS) isoenzymes GLS1 and GLS2 catalyze the first step of glutaminolys.

Objective Glutaminase (GLS) isoenzymes GLS1 and GLS2 catalyze the first step of glutaminolys.

Methods : We measured reactive oxygen species (ROS) levels, lipid peroxidation, and mitochondrial mass and polarization by flow cytometry, production of interleukin 2 (IL-2) by dual-luciferase assay , and by real-time polymerase chain The CpG DNA methylation reaction system for measuring Il

Methods We measured reactive oxygen species (ROS) levels, lipid peroxidation, and mitochondrial mass and polarization by flow cytometry, and production of interleukin 2 (IL-2) by dual-luciferase assay, measured by real-time polymerase chain Il2 CpG DNA methylation reaction syste.

As a result GLS2 , but not GLS1 , reduced ROS levels and lipid peroxidation in T cells and restored mitochondrial functio.

Conclusions These findings suggest that GLS2 plays a critical role in IL-2 production by CD4+ T cells by supporting antioxidant defense , and they provide a novel approach to correct T cell production of IL-2 in SL.

Hisada R, Yoshida N, Orite SYK, Umeda M, Burbano C, Scherlinger M, Kono M, Krishfield S, Tsokos .
Role of Glutaminase 2 in Promoting CD4+ T Cell Production of Interleukin-2 by Supporting Antioxidant Defense in Systemic Lupus Erythematos.
Arthritis Rheumat.
2022 Jul;74(7):1204-121 doi: 11002/a.
4211 Epub 2022 May 1 PMID: 3525400 Leave a comment here