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    Home > Biochemistry News > Biotechnology News > A sample of points in thin layer separation.

    A sample of points in thin layer separation.

    • Last Update: 2020-10-27
    • Source: Internet
    • Author: User
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    point sample in thin layer analysis separation
    First, dot sample position: 1, up-line unfolding method point sample is generally 4 to 5 cm away from the lower end of the thin layer.2, the down-line unfolding method point is generally 6 to 8 cm away from the upper end of the thin layer.3, two-way unfolding method sample should be located from the right side of the thin layer 5cm and from the bottom edge 5cm straight intersection.2, dot note: 1, samples are best to use volatile
    organic
    solvent dissolved, should not be water solution, because the water molecule and adsorbent interaction force is weak, when the water molecule occupies the active position of the adsorbent surface, will reduce the activity of the adsorbent, and make the speck diffuser.2, dot sample quantity should not be too much, otherwise it will reduce the migration rate, generally a few to tens of micrograms.3, the diameter of the origin should be controlled within 2mm. To do this, it must be divided into points, edge sample edges with cold, hot air alternately blow-dried.4, thin sheet in the air can not be placed for too long, otherwise it will be reduced activity due to moisture absorption.
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