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    Home > Biochemistry News > Biotechnology News > A solution of deoxynucleoside triphosphate (dNTP) in the preparation of a commonly used storage fluid.

    A solution of deoxynucleoside triphosphate (dNTP) in the preparation of a commonly used storage fluid.

    • Last Update: 2020-10-26
    • Source: Internet
    • Author: User
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    .

    digestion factor (ε)"L/(mol?cm)"

    deoxynucleoside triphosphate (dNTP) solution

    " (preparation method)

    dissolves each dNTP in water to a concentration of 100mmol/mol/left L or so, with a trace>-shifter to absorb 0.05mol/l Tris alkali respectively adjust the pH 7.0 of each dNTP solution (with pH test paper test), the middle and rear of each of the DNTP solution each take a copy for appropriate dilution, read light at wavelengths given in the table belowdensity meter to calculate the actual concentration of each dNTP, and then dilute with water to a final concentration of 50 mmol/L dNTP, divided into small parts stored at -70 degrees C.

    base

    "nm"

    A

    259

    .

    1.54×104

    .

    G

    22" 53

    1.37×104

    ."

    C

    271

    9.10×103

    260

    7.40×103

    -color cup light diameter is 1cm, absorbance s.m

    (responsible editor: King Han Kunlun)

    .
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