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deoxynucleoside triphosphate (dNTP) solution " (preparation method) dissolves each dNTP in water to a concentration of 100mmol/mol/left L or so, with a trace>-shifter to absorb 0.05mol/l Tris alkali respectively adjust the pH 7.0 of each dNTP solution (with pH test paper test), the middle and rear of each of the DNTP solution each take a copy for appropriate dilution, read light at wavelengths given in the table belowdensity meter to calculate the actual concentration of each dNTP, and then dilute with water to a final concentration of 50 mmol/L dNTP, divided into small parts stored at -70 degrees C. | "nm" | . digestion factor (ε)"L/(mol?cm)" |
A
259
1.54×104
G
22" 53
1.37×104
."
C
271
9.10×103
260
7.40×103
-color cup light diameter is 1cm, absorbance s.m
(responsible editor: King Han Kunlun).