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    Home > Biochemistry News > Plant Extracts News > A Straightforward Protocol for Electro-transformation of Phytophthora capsici Zoospores

    A Straightforward Protocol for Electro-transformation of Phytophthora capsici Zoospores

    • Last Update: 2020-11-19
    • Source: Internet
    • Author: User
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    Genome sequencing combined with high-throughput functional analyses has proved vital in our quest to understand oomycete–plant interactions. With the identification of effector molecules from
    Phytophthora
    spp. we can now embark on dissecting the mechanisms by which effectors modulate host processes and thus ensure parasite fitness. One of the key limitations, however, is to genetically modify
    Phytophthora
    and assess gene function during parasitism. Here, we describe a straightforward protocol that allows rapid transformation of
    Phytophthora capsici
    , an emerging model in oomycete biology.
    P. capsici
    is a broad host range pathogen that can infect a wide variety of plants under lab conditions making it a suitable model for detailed studies on oomycete–host interactions. This protocol relies on electroporation-assisted uptake of
    DNA
    in to motile zoospores and allows the rapid identification and characterization of genetically stable transformants.
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