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    Home > Biochemistry News > Biotechnology News > A system OFHIT2 that can simultaneously implement drug-induced genome editing and transcription activation.

    A system OFHIT2 that can simultaneously implement drug-induced genome editing and transcription activation.

    • Last Update: 2020-08-11
    • Source: Internet
    • Author: User
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    The correct expression of genes in space-time and its precise and orderly regulation are the premise and basis for the orderly conduct of important physiological processes such as cell growth, proliferation, differentiation, aging and apoptosis.
    , and by controlling the way, precise manipulation of gene function from the molecular level is of great importance for more accurate analysis of biological mechanisms and more controlled and safer clinical applications.
    Wang Yu Research Group of the Institute of Zoology of the Chinese Academy of Sciences is committed to the development of novel and useful molecular and cellular biology tools through chemical biology.
    researchers propose to create a drug-induced CRISPR/Cas9 technology (HIT, drug Hybrid Scrispur CRISPR/Cas9 Technologies) that manipulates functional events more flexibly and accurately from the molecular level by grafting the estrogen receptor element (ERT2) on the CRISPR/Cas9 system so that its approved position within the cell is regulated by the small molecule drug 4-hydroxytamoxifen (4OHT).
    researchers from the transcription activation system, after a series of optimization, transformation, comparison of a variety of technical solutions, the establishment of a low-end, high-efficiency drug induction system (HIT-SunTag).
    on this basis, using different lengths of sgRNAs selectively guide the characteristics of Cas9 protein binding and shearing target DNA, a system for drug-induced genome editing and transcription activation (HIT2, meaning "one arrow and double carving").
    further, they compare back-to-back with existing drug induction systems, including Zhang Feng's Split-Cas9 system, David Liu's intein system, etc., and the HIT-SunTag and HIT2 systems present advantages such as lower base, higher efficiency, higher drug selection specificity, reversibility and rapid response. the establishment of these systems
    provides a powerful technical tool for more flexible, diverse, accurate and controlled editing and regulation of gene functions to achieve higher space-time "resolution" in the analysis of key biological events, and thus provides more potential for clinical applications by combining the advantages of small molecule drugs and gene therapy.
    research in the multimode drug inducrec CRISPR/Cas9 devices forrisonal activation and genome editing, published online in Nucleic Acids Research. Lu Jia, Ph.D. Zhao Chen, Zhao Yingze and Zhang Jingfang, master's students of the
    Animal Institute, are the co-first authors of the paper, and researcher Wang Yu is the author of the paper communication.
    research work has been supported by the Ministry of Science and Technology's National Major Scientific Research Program, the National Natural Science Foundation of China, the "100-person Program" of the Chinese Academy of Sciences, and the Open Project Fund of the National Key Laboratory for Stem Cell and Reproductive Biology.
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