Acetic acid

Acetic acid

Pseudolaric acid B

【Synonyms】Gumboacetic acid

【Structural formula】

【Molecular formula and molecular weight】C ₂₃ H ₂₈ O ₈ ;432.

[Source] Dry root bark or near-root bark of Pseudolarix amabilis (Nelson) Rehd

【Properties】White crystals

This product was dissolved in chloroform , acetone , methanol and the like

Melting point: 127～128℃ ( ether - petroleum ether ), 145～146℃ ( acetone )

【Purity Check】

TLC

1.

Developing solvent of toluene - ethyl acetate - formic acid (14:4:0.

Check the 10% sulfuric acid ethanol solution, heat it at 105°C until the spots appear clear, and check under sunlight and ultraviolet light (365nm)

2.

Eluent ether - petroleum ether (30 ~ 60 ℃) - acetic acid (2:1: 0.

Check the 5% vanillin sulfuric acid solution, heat it at 105°C until the spots appear clear, and check under sunlight

High performance liquid chromatography

Chromatographic column Alltima C ₈ , 5um (4.

The mobile phase of methanol -1% acetic acid solution (50:50), 1ml / min

Detection 260nm

【Structural identification】258,228(sh)

2952, 1743, 1712, 1645, 1610, 1442, 1371, 1279, 1244, 1165, 1072, 951

FAB-MS m/z: 455[M+Na] ⁺ , 432[M] ⁺ , 387, 373, 355

¹ H-NMR (CDCl ₃ ) δ: 7.

¹³ C-NMR (CDCl ₃ ) δ: 33.
1(C-1), 24.
1(C-2), 51.
8(C-3), 90.
1(C-4), 30.
5(C-5), 27.
5(C-6) ), 134.
4(C-7), 141.
5(C-8), 20.
0(C-9), 55.
0(C-10), 83.
6(C-11), 28.
2(C-12), 144.
3(C-13) , 121.
5(C-14), 138.
2(C-15), 127.
6(C-16), 12.
3(C-17), 169.
0(C-18), 167.
7(C-19), 172.
6(C-20), 172.
5(C-21), 21.
5(C-22), 49.
1(C-23)
.

【Storage】Dry and airtight
.

Related link: Adenosine