ACS Nano of Wang Jianhao and Jiang Pengju team of Changzhou University: wound healing of diabetic patients infected with biofilm based on pH responsive antimicrobial gel

The wound healing of diabetic patients is slow, which makes the wound easy to be infected In addition, the high sugar content in the blood of diabetic people and the local anoxia caused by their own vascular disease will accelerate the bacterial reproduction and form a biofilm at the wound site The formation of biomembrane can induce the occurrence of chronic inflammation, so as to prevent the normal healing cascade reaction of wound (hemostasis, inflammation, proliferation and maturity), and eventually make the wound not heal, and cause serious health problems such as amputation Therefore, it is very important to develop a kind of bioactive material which can quickly eliminate the biofilm and promote the wound healing cascade reaction for the management and rapid healing of diabetic patients Recently, Wang Jianhao and Jiang Pengju of Changzhou University published a research paper entitled "pH switchable antimicrobialnanofiber networks of hydraulic eradicate biofilm and rescue stalled heating inchronic sound" on ACS Nano (if = 13.903), an international top academic journal of chemistry and materials (DOI: 10.1021 / acsnano 9b05608) Changzhou University is the first unit of the paper, Professor Jiang Pengju is the first corresponding author, Associate Professor Wang Jianhao and 2016 master student Zhao Yuan are the co authors of the paper, Dr Yang Baozhu participated in the theoretical calculation of the paper, associate professor Qiu Lin and 2017 master student Du Xuan participated in some experiments Prof Wang Jianhao is mainly engaged in nano biological analysis, surface modification of nano materials and its application in tumor diagnosis and bacterial detection In 2014, he was supported by the first 863 Program young scientists program In addition, it has also completed 2 projects of NSFC, 1 project of NSFC overseas cooperation fund with scholars from Hong Kong and Macao, presided over key provincial R & D plans, natural funds, Changzhou social development, international cooperation projects and many projects entrusted by enterprises In the past five years, he has published nearly 40 papers (including 33 first authors and corresponding authors) collected by SCI in international famous journals such as ACS Nano, nanoscale (front and hot papers), small (back and back papers), sensor actuator B: chem., anal.chim Acta, and authorized 11 invention patents He served as the director of Changzhou society of Biochemistry and molecular biology, society of Biomedical Engineering, etc he was the reviewer of many international journals, and served as the guest editorial board member of SCI journals frontier in Chemistry (zone 2, impact factor 3.78) In 2014, it was selected as the provincial "six talent peaks" class C project funding, in 2016, it was selected as the third level training object of Jiangsu 333 project, and in 2018, it was selected as the vice president of science and technology of the provincial entrepreneurship and innovation program In 2016-2018, he was rated as the first level post of incentive performance allowance of Changzhou University and the first associate professor of the first level post since the reform of performance pay In 2018, he won the second prize of Jiangsu Education and research achievements (ranking first) Prof Jiang Pengju received his Ph.D from Oxford University in 2007 After that, he worked as a postdoctoral researcher in the Department of biochemistry of Oxford University, mainly studying metal antitumor drugs and fluorescent molecular probes in the life system During his study abroad, he mainly engaged in structural biology research, using 950 MHz A series of biophysical methods, such as internal super field solution NMR, have systematically studied the protein interactions related to cell migration, revealed various protein structures, interaction modes and protein stability in the complex life process After returning to China in 2010, a research group was set up in Changzhou University to carry out research in biology, materials, drugs and other related fields They have successively worked in molecular cell, EMBO J, J am Chem SOC., ACS Nano, nanoscale, More than 50 SCI papers have been published in the top international journals of Biochemistry and chemistry, such as coordination chemistry reviews, with a cumulative impact factor of 250, cited more than 2000 times, applied for more than 50 patents, presided over 7 national and provincial projects, and participated in 863 projects of the Ministry of science and technology and key projects of the Foundation Committee Frontier scientific research achievements: biofilm infection and wound healing of diabetes based on pH responsive antibacterial hydrogels, with the improvement of people's living conditions, the number of diabetic patients is increasing According to the eighth edition of the global diabetes map released by the International Diabetes League the year before last, the number of diabetic patients aged 20-79 in the world has increased by 274 million over the past 17 years, compared with 151 million, and it is estimated that the number will continue to increase For normal people, the wound can heal itself, but for diabetic patients, the wound healing is slow, or even not, which makes the wound more susceptible to infection, leading to serious health problems When trauma occurs, diabetic patients lose the ability to respond to the wound due to the abnormal cell performance around the wound, and the ability of leukocyte sterilization is very weak, which will make the wound susceptible to bacterial infection Moreover, the high glucose content in the blood and the local anoxia caused by the disease of blood vessels will accelerate the bacterial reproduction, make the wound infection more serious and form the biofilm Biomembrane infection can induce chronic inflammation and prevent the normal coordination process of wound healing cascade, which is an important reason for the long-term wound non healing in diabetic patients Therefore, it is of great significance to develop a kind of bioactive material which can quickly eliminate biofilm and promote wound healing The hydrogels formed by peptides have excellent biocompatibility and are expected to become potential environmental friendly materials, which has attracted the interest of researchers Based on previous studies, we designed an acidic pH activated broad spectrum antibacterial eight peptide (IKFQFHFD), which has good biocompatibility under the condition of neutral pH and can form a supramolecular hydrogel drug loading system through self-assembly Through transmission electron microscope, it can be seen that the nanofibers have good dispersibility from the micro morphology level, and the diameter of nanofibers is about tens of nanometers The hydrogel showed acid pH (the pathological environment pH=5.5 of infected chronic wounds) The antibacterial activity of Staphylococcus aureus (MRSA) incubated with hydrogels in 96 well plates was detected The authors found that hydrogels exhibited strong antibacterial activity under acidic conditions, and the bacteriostatic efficiency could reach 80% at pH=5.5 The circular dichroism shows a typical β - fold secondary structure The rheological properties of the colloid were characterized by rheological tests The results showed that the gel state could be maintained in a certain frequency range The MTT toxicity test and the Live/Dead test co cultured with HUVEC cells were carried out The survival rate of 48 h cells can still reach 83%, which proves that the peptide hydrogel has good biocompatibility Fig 1 Characterization and characterization of hydrogels (source: ACS Nano) In addition, the authors developed hydrogel networks loaded with cypate (photothermal agents) and proline (procollagen) drugs In vitro experiments showed that the hydrogel loaded with drugs showed an acidic (pH=5.5) responsive release curve, and the Cypate and proline release rates were all about 60%, and the pH responsive composite hydrogel achieved synergistic elimination of biofilms and subsequent endothelial cell proliferation In addition, animal experiments showed that nanofiber hydrogel network could completely cure MRSA biofilm infected wounds in diabetic mice within 20 days, showing its great potential as a clinical chronic wound dressing Fig 2 Schematic diagram of pH responsive antibacterial gel for eliminating biofilms and rescuing chronic wound healing Stagnation (source: ACS Nano) It is reported that the Wang Jianhao and Jiang Pengju team has made many achievements in the field of nanomaterial biological application in recent two years Relevant achievements have been successively published in nanoscale 2018, 10, 132 (cover papers and hot papers), small 2018, 14, 1802745 (back cover papers), ACS sustainable chem Eng 2018, 6, 4504, ACS appl Mater Interfaces 2019, 11, 23093 and other journals Nowadays, people and scientific research have been paid more and more attention in the economic life China has ushered in the "node of science and technology explosion" Behind the progress of science and technology is the work of countless scientists In the field of chemistry, in the context of the pursuit of innovation driven, international cooperation has been strengthened, the influence of Returned Scholars in the field of R & D has become increasingly prominent, and many excellent research groups have emerged in China For this reason, CBG information adopts the 1 + X reporting mechanism CBG information website, chembeangoapp, chembeango official micro blog, CBG information wechat subscription number and other platforms jointly launch the column of "people and scientific research", approach the representative research groups in China, pay attention to their research, listen to their stories, record their demeanor, and explore their scientific research spirit.