AdTp: elastin-like polypeptide enhancement adeno-associated virus in Zongchao Han's research group at the University of North Carolina is expressed under pre-storage neutralizing antibody conditions

Gene therapy is a treatment method

Recently, Zongchao Han's research group from the University of North Carolina used elastin-like polypeptides (ELPs) to encapsulate AAV to form nanoparticles (ELP-AAV), which enhanced the delivery of AAV and gene expression

[Figure 1] (A) Comparison of ELP-AAV entry into cells and ordinary AAV into cells (B) Morphological comparison of ordinary AAV and ELP-AAV (C) Determination of ELP at the junction site (D) of ELP in different serotypeSAAV by MALDI-TOF Transduction efficiency of ELP-AAV on the cell line

【Figure 2】 Transduction efficiency of cell lines under neutralizing antibody conditions for different ELP-AAV and ordinary AAV

【Figure 3】PBS stability (B) of ELP-AAV (A) And stability of serum neutralizing antibodies (C) degradation after entering cells

[Figure 4] Neutralizing antibody titers initiated by ELP-AAV and ordinary AAV(A) injection mice and (B-D) ELP-AAV and ordinary AAV on mouse models of pre-injected neutralizing antibodies are reported gene expression and organ distribution

[Figure 5] ELP-AAV and ordinary AAV (A-C) reported gene expression and organ distribution on mice with pre-stored AAV immunity

WILEY

Thesis Information:

Elastin-Like Polypeptides Facilitate Adeno-Associated Virus Transduction in the Presence of Pre-Existing Neutralizing Antibodies

Kai Wang, Min Zheng, Charles Askew, Xintao Zhang, Chengwen Li, Zongchao Han*

Advanced Therapeutics

DOI:10.

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