Advances have been made in the study of microbial synthetic biomedical materials.

Biotechnology Channel News: There is a kind of microorganisms like to live in a high-salt environment on Earth, extreme living environment makes this kind of salt-obsessed microorganisms evolved a special ability to survive.
study of salt-addicted microorganisms not only provides important enlightenment for exploring the extreme adaptation mechanism of life, but also provides the possibility for the use of its special functions and metabolites.
The Institute of Microbiology of the Chinese Academy of Sciences has been engaged in basic research on the genetic mechanisms of extreme saline-obsessed germs (such as genomic replication and CRISPR function) on the one hand, and on the other hand, the basic research on the application of biodegradable plastics for the synthesis of salt-containing microorganisms has been carried out for a long time.
Through more than 10 years of systematic work, the team has systematically articulated the key enzymes, key pathways and associated regulatory factors involved in the synthesis and degradation of the biodegradable plastic PHBHV, represented by the Mediterranean salt-rich bacteria, and used metabolic engineering to increase the yield of PHBHV.
the past two years, the Team has made a series of new advances in basic research on the synthesis of high value-added biomedical materials using salt-addicted germs.
Mediterranean salt-rich bacteria can use a variety of low-cost carbon sources to efficiently synthesize 3HV monolith ratio constant (-10 mol%) PHBHV, and can be easily extracted by water, so it has important industrial development potential.
in order to solve the problem of the relatively single type of PHBHV synthesis of the bacteria, the Chinese team used the bacteria as a cell factory to synthesize a series of R-PHBHV and O-PHBHV with different monolith polymerization methods through fermentation engineering technology.
3HV monomer content of the polymer was increased from 10 mol% to 60 mol, enriching the material properties of the saline bacteria PHBHV, such as the fracture elongation of the material increased from 5% to 508%.
,O-PHBHV,,,,Biomacromolecules, 2015,16:578-588。
To further advance the high value-added application of PHBHV in regenerative medicine for salt microbial synthesis, the team systematically studied the degradation properties and bioserance of materials in simulated body fluids (PBS containing lipases) and rabbits.
Compared to PLA (FDA-approved biomedical polymer materials) and reu-PHB and Reu-PHBHV from bacterial sources, PHBHV from salt-obsessed microbial sources is more conducive to the attachment and proliferation of fibroblasts and bone cells, demonstrating better cell compatibility.
PHBHV synthesized by salt-eating microorganisms degrades faster under rabbit skin than in simulated body fluids, and different materials exhibit different degradation speeds.
, O-PHBHV with micron holes on the membrane surface degrades the fastest, the first PHA material reported to degrade faster than PLA.
also found that when implanted under the skin of rabbits, the salt-eating bacteria PHBHV caused only a slight inflammatory response compared to bacterial materials and PLA.
therefore, PHBHV synthesis of salt-obsessed microorganisms has a variety of degradation speeds and good bio-compatibility, suggesting that these materials will have broad application prospects in different biomedical fields.
The latest developments have opened up new directions for the further development of extreme microbial resources and the synthesis of high value-added biomedical materials, and will also enhance the medical value of salt-eating microorganism PHBHV in the fields of scar healing, cartilage repair, nerve repair, etc.
Jing (Associate Researcher, 2015 Member of the Chinese Academy of Sciences Youth Promotion Association) is the first author of these two papers and the author of the newsletter to Huawei.
research has been funded by the National Natural Science Foundation of China (key projects and surface projects) and the Chinese Academy of Sciences Youth Innovation Promotion Association.
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