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Biotechnology Channel News: Stem cells are a class of cells with self-renewal ability and multi-way differentiation potential, showing attractive prospects in biomedical fields such as regenerative medicine, drug screening and toxicology.
the number of stem cells available through the current stem cell separation culture technology is very small, far from meeting clinical needs, so in-body large-scale amplification of cultured stem cells is an urgent problem to be solved.
the paper briefly describes the characteristics of various bioreators suitable for stem cell culture, as well as the research application of suspended bioreator systems in different types of stem cell groups.
, several key parameters in the process of using bioreator to culture stem cells are expounded, which will provide reference for stem cell culture and research.
stem cells (stem cells, SC) are a class of undifferentiated, immature special cells present in the body, with the potential of self-renewal, proliferation and multi-way differentiation, can be separated, amplification and cryoprotective preservation in vitro, and can be induced to different tissue cells and organs under appropriate conditions, with potential regenerative functions.
At present, embryonic stem cells (ESCs), induced pluripotent stem cells,iPSCs, and other stem cells offer attractive prospects in areas such as regenerative medicine, drug screening, introphysiological toxicology, and so on, giving great hope to the scientific and medical communitys.
, however, in general, the number of stem cells available from the feeder is very small.
Even in abundant bone marrow, there is only one hematopoietic stem cell (haematopoietic stem cell, HSC) per 10,000-15,000 bone marrow cells, and the content of stem cells in human and animal skin is only 7% to 8%.
many cell therapies, the amount of cells needed to treat an adult patient is far greater than the amount of cells available from the supply.
example: patients with myocardial infarction need 1 x 109 to 2 x 109 heart muscle cells to replace damaged cardiomyopathy tissue.
islet transplant, a patient weighing 70 kg needs 1.3 x 109 islet beta cells.
Therefore, in order to push stem cells to practical applications such as clinical application and industrialization, one of the problems that urgently needs to be solved is how to expand these stem cells on a large scale in in-body, so that stem cell products can meet the requirements of clinical treatment in quantity.
At present, the bioreactor provides an effective method for stem cell culture, using bioreactor culture stem cells can achieve large-scale amplification, and can effectively maintain the undifferentiated characteristics of stem cells, and thus meet the requirements of clinical cell therapy.
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