Agarose gel electrophoresis recycles PCR products.

related topics .One. PrincipleThe separation and recovery of
pieces of DNA is an important technique in the operation of
gene
engineering
such as the collection of specific enzymes
slices
pieces
for cloning or preparation of probes, recovery of
PCR
products for re-identification, etc. The two most important technical indicators in the recovery experiment are
purity and recovery rate: the former does not meet the standard will seriously affect the later enzyme cutting
, connection, marking and other enzymes involved in the reaction;
experiment used V-GENE's DNA gel recovery
reagent
box on the principle that the gel block in the gel melt liquid (Buffer
DE-A) was rapidly melted and released DNA. After adding a high-ion sequence solution (Buffer DE-B),
fragments are selectively adsorbed to the silica membrane. After Buffer W1, Buffer W2 washing removes impurities and high
concentration salt ions that remain on the silica membrane, the pieces of DNA adsorbed to the silica membrane are eluted by trace amounts of water or Eluent and can be used in a variety of
moleculesbiology experiments.two. Materials and Methods
1 Materials
PCR products (not purified)
2 instruments, appliances
electrophoresis meters, electrophoresis tanks, gel sample combs, microwave ovens, desktop
centrifuges
,

,
thermostat
incubator (55-65 degrees C),
1.5 ml centrifugal tube
3 reagents
1×TAE electrophoresis buffer; ethyl bromide ingot solution (EB)
.