β-Agonist Residue Analysis Technology

1.

At present, the animal-derived matrices involved in the analysis of β-receptor agonists mainly include urine, liver, kidney, blood, muscle, etc.

(1) Extraction method

The way the sample is processed will affect the final result of the β-agonist residue analysis

β-receptor agonists, especially phenolic drugs such as salbutamol , often form conjugates in biological samples, such as binding with glucuronidase and sulfate proteins

Haasnoot et al.

For drugs that exist in a free state, buffers or organic solvents can be used directly for extraction.

(2) Purification method

The commonly used purification methods for the analysis of β-agonist residues include liquid-liquid partitioning (LLP), solid phase extraction (SPE), matrix solid phase dispersion extraction (MSPD) and so on

1) Liquid liquid partition (LLP)

When using LLP to purify Clenbuterol and other β-receptor agonists in the extract, it is often necessary to adjust the pH to above 10, and then use an organic solvent such as ethyl acetate for extraction

The ITP keeps the analyte away from the liquid-liquid interface, and then is measured by capillary zone electrophoresis-electrospray mass spectrometry

2) Solid phase extraction (SPE)

β-receptor agonists are mainly purified by cation exchange and reversed-phase SPE columns

Whaites et al.

Nie Jianrong and others have established clenbuterol, ractopamine, salbutamol, cimaterol, mabuterol, tobuterol, bambuterol, maspenterol, sebuterol, zipa in animal urine.

Zhang et al.

In order to reduce the influence of matrix effect on liquid chromatography-mass spectrometry (LC-MS) quantification, Van Hoof et al.

3) Matrix solid-phase dispersion (MSPD)

The most commonly used MSPD adsorbent is mainly C ₁₈

4) Supercritical fluid extraction (SFE)

With the development of new purification technologies, some new technologies such as supercritical fluid extraction (SFE), etc.