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Agrobacterium-Mediated Transformation of Cotton

 Last Update: 2021-03-14
 Source: Internet
 Author: User

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There are many methods and techniques that can be used to transfer foreign genes into cells. In plant biotechnology,
Agrobacterium
-mediated transformation is a widely used traditional method for inserting foreign genes into plant genome and obtaining transgenic plants, particularly for dicot plant species.
Agrobacterium
-mediated transformation of cotton involves several important and also critical steps, which includes coculture of cotton explants with
Agrobacterium
, induction and selection of stable transgenic cell lines, recovery of plants from transgenic cells majorly through somatic embryogenesis, and detection and expression analysis of transgenic plants. In this chapter, we describe a detailed step-by-step protocol for obtaining transgenic cotton plants via
Agrobacterium
-mediated transformation.

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Agrobacterium-Mediated Transformation of Cotton

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