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Agrobacterium tumefaciens Chemotaxis Protocols

 Last Update: 2020-11-13
 Source: Internet
 Author: User

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Directed movement in response to chemical attractants is of crucial importance to
Agrobacterium tumefaciens
. Motility is a determinant of rhizosphere competence (
1
), and chemotaxis a conditional requirement for virulence (
2
,
3
).
A. tumefaciens
is attracted toward a variety of sugars typical of plant exudates (
4
). Responses to nanomolar amounts of phenolic wound exudates are chiefly determined by the Ti-plasmid encoded virulence genes
vir
A and
vir
G (
5
,
6
). The sensitivity of these responses indicates that they play a role in the biology of
A. tumefaciens
. Thus, the attraction to sugars can partly explain the organism’s prevalence in the rhizosphere (
7
) and the highly sensitive response to phenolics, may help to guide
A. tumefaciens
to wounded plant cells (
8
,
9
).

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