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    Home > Biochemistry News > Plant Extracts News > Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction

    Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction

    • Last Update: 2021-01-16
    • Source: Internet
    • Author: User
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    Thermal asymmetric interlaced polymerase chain reaction (TAIL-
    PCR
    ) is an efficient tool for the recovery of
    DNA
    fragments adjacent to known sequences. A protocol is presented for the amplification of genomic sequences flanking DNA (T-DNA or transposon) insertions using the TAIL-PCR method. The amplified products are suitable as templates for direct sequencing, or for cloning in vectors. Examples are given in use of the protocol for T-DNA tagging in rice using the pCAMBIA binary vectors.
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