It was learned on April 20 that " an efficient and high-definition fluorescence microscopy method for observing the growth of plant pollen tubes in pistils " completed by researchers such as Xu Yuanqing and Luo Zhonglai of the South China Botanical Garden of the Chinese Academy of Sciences was authorized by the national invention patent
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The fluorescence microscopy method of the invention greatly weakens the phenomenon of the luminescence of the pistil itself, and reduces the interference to the observation of pollen tubes when the magnification is large, so that it is easy to obtain a high-definition image of the growth of the pollen tube under high magnification.
Image, the rendering of details is very good
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The fluorescence microscopy method of the invention greatly shortens the softening and transparent time to several hours, and the dyeing time is also shortened from several hours to several minutes in the prior art, so that the observation experiment can be completed in one day, which greatly improves the At the same time, the use of high-concentration NaOH solution is avoided, and the size of the material can remain in the original state during the softening and transparent process, so there will be no water loss and shrinkage, and no rehydration treatment will be required
.
