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July 6 was informed that by the Chinese Academy of Sciences South China Botanical Garden Zhang Xinhua and other researchers completed "an efficient method of inducing the occurrence and regeneration of hybrid sandalwood cell embryos" has been granted a national invention patent (patent number: ZL201610038247.1).
the invention selected the fine cultivated hybrid sandalwood young stem as the outer implant, using the sub-sterilization procedure for the surface disinfection of the outer implant can obtain a high success rate.
sterile exosomes that have successfully disinfected are cultured on a culture containing 2,4-D of germ-based healing tissue, induces fibrous germ-like healing tissue, and then transfers to somatic cell embryo-induced, mature, germination, plant regeneration culture medium, and can be regenerated plants after 3 months.
the characteristics of short breeding cycle, large number and high stability, solve the problem of low reproduction efficiency in the breeding seedlings of hybrid sandalwood tissue, and will provide seedling technology for the rapid reproduction of hybrid sandalwood seedlings, as well as the regeneration method for efficient gene conversion.
the invention has the advantages of strong operation, reproduction efficiency and high application value.
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