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    Home > Active Ingredient News > Study of Nervous System > Analysis of exosomal microRNA expression profile in urine of AMEM Alzheimer's disease 5XFAD mouse model

    Analysis of exosomal microRNA expression profile in urine of AMEM Alzheimer's disease 5XFAD mouse model

    • Last Update: 2021-10-11
    • Source: Internet
    • Author: User
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    BACKGROUND Alzheimer's disease (Alzheimer's disease, AD) is a refractory and irreversible neurodegenerative disease, the pathogenesis of which is still unclear

    .

    Therefore, before the deposition of amyloid-β plaques (Aβ), the identification of candidate biomarkers is of great significance for the early intervention of AD
    .

    Researchers from the Pathology Platform Task Force of the Institute of Medical Experimental Animals of the Chinese Academy of Medical Sciences have determined the microRNA (miRNA) in the urine exosomes of the early Alzheimer mouse model to find markers that can identify AD in the early stage
    .

    In this paper, a 1-month-old 5XFAD mouse model and its littermate control group in the early stage of Aβ plaque formation were selected to collect exosomes in urine and analyze the differential expression of miRNAs in them, using bioinformatics methods to analyze the differences The expressed genes were displayed, and at the same time, KEGG pathway analysis and GO enrichment analysis were performed on the differentially expressed genes screened out
    .

    The results showed that: 48 differentially expressed miRNAs (18 up-regulated, 30 down-regulated), of which miR-196b-5p, miR-339-3p, miR-34a-5p, miR-376b-3p, miR-677-5p and miR -721 -6 miRNAs are predicted to show gene targets and important signal pathways closely related to the pathogenesis of AD
    .

    The above conclusions were verified by ddPCR and a consistent conclusion was obtained
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     Figure 1 Workflow A of miRNA microarray analysis of urine exosomes in 5 XFAD Alzheimer's disease mouse models, sampling and isolation of urinary exosomes, and miRNA microarray evaluation
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    B.
    A block diagram showing the distribution of microarray data samples

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    C.
    Correlation graphs of extraurinary body samples in the 5XFAD model and the fat control group

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    At the end of this article, combined with the molecular analysis of miRNA target interaction network, it is used to analyze the 5XFAD mouse AD model of urinary exosomes miRNA expression profiling
    .

    Visualize the network of experimentally verified target genes and their miRNA functional annotations, and the node size reflects the degree of connection
    .

    Figure 2 Through clustering, scatter plot, KEGG pathway analysis and regulatory network analysis, the expression of urinary exosomal miRNA is different in the 5XFAD mouse model before Aβ plaque deposition
    .

    These exosomal miRNAs represent potential non-invasive biomarkers and can be used as reliable non-invasive sources of early AD biomarkers, and can be used in clinical applications to prevent the development of AD in the future
    .

    Figure 3 Urine exosomes miRNA chip analysis of differentially expressed candidate miRNAs in the 5XFAD mouse AD model Med.
    2021; https://doi.
    org/10.
    1002/ame2.
    12175 [Health Sciences] About the journal "Animal Models and Experimental Medicine (English)" (Animal Models and Experimental Medicine, AMEM) by the Chinese Society of Experimental Animals, Chinese Medicine Co-sponsored by the Institute of Medical Laboratory Animals of the Academy of Sciences, and published in cooperation with the internationally renowned publishing group Wiley

    .

    AMEM has been indexed by DOAJ, PMC, CSCD, MEDLINE, ESCI, with readers all over the world including China, the United States, India, the United Kingdom, Germany, Japan, and Canada
    .

    AMEM focuses on experimental animal science basic and applied research, focusing on experimental animal species resources, human disease animal model resources, comparative medical resources, etc.
    , displaying the latest academic, theoretical, technical, and application results of experimental animal science and experimental medicine, as well as the life of experimental animals Experimental research in science, medicine, biology, pharmacy, veterinary science and other cross-disciplines, expand the research and development of laboratory animal related equipment and materials, laboratory animal management and legal standards, laboratory animal welfare ethics and other new ideas.
    It is a book that focuses on interdisciplinary, An English professional journal that focuses on the transformation of results and leads the development of experimental animal science and experimental medicine

    .

    AMEM full-text open access (Open Access), to ensure that each article can be read, downloaded and shared by readers all over the world for free
    .

     AMEM adopts ScholarOne online manuscript processing system and a peer review mechanism (Single-Blind Peer Review).
    Authors can submit and view articles anytime, anywhere

    .

    AMEM adopts free format submission to reduce the pressure of authors to contribute and promote the dissemination of scientific research results
    .

    AMEM can exempt APC for high-quality manuscripts
    .

    AMEM welcomes contributions from industry experts and scholars! ▼A wonderful review of the past ▼Discussion on the mechanism of retinal injury and retinal stem cell activation in vertebrate models Phase 2 is online! Recruitment | "Animal Model and Experimental Medicine (English)" (AMEM) young and middle-aged editorial board recruitment content | AMEM layout | AmandaWiley promotes world progress and development with scientific research and education
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    By providing publications, platforms, and services, we help students, researchers, universities, and companies achieve their goals in an ever-changing world
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    For more than 200 years, we have always brought continuous and stable performance to all stakeholders
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