Analysis of genetically modified plant gene expression using GUS fluorimetry
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Last Update: 2020-11-22
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Source: Internet
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Author: User
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A fluorimetric assay method for the analysis of beta-glucuronidase (GUS) reporter gene expression in genetically modified plants is described. Optimization of this method for woody plants and a statistical approach suitable for comparisons of gene expression in different transformants or tissues of the same plant is described. Example data from elm (
Ulmus procera
) SR4 regenerant plants, shown to be genetically modified by
PCR
and
DNA
-DNA hybridizations, in which higher GUS expression levels are found in stems than in leaves demonstrates the utility of this approach.
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