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    Home > Angelw: a new subcellular localization probe -- BODIPY type light cage molecule

    Angelw: a new subcellular localization probe -- BODIPY type light cage molecule

    • Last Update: 2019-02-17
    • Source: Internet
    • Author: User
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    Cell is the basic unit of life structure, which consists of cell membrane, cytoplasm and nucleus The cytoplasm inside the cell membrane and outside the cell nucleus is not a uniform structure There are many suspended particles called organelles, including mitochondria, lysosomes, Golgi bodies, etc these organelles are responsible for the normal metabolism of cells At present, people mainly use small molecules to regulate the physiological process of these organelles to study their structure and function, but most of them can not achieve accurate positioning Therefore, the development of molecules with accurate localization performance has been a hot topic for scientists In addition, when a molecule enters a specific organelle, how to precisely control the regulatory process is also a problem to be considered in the molecular design process Recently, the Roy weininin research group of the school of plant science and food safety of Tel Aviv University in Israel reported a kind of photocage molecules based on boron fluoride fluorescent dye (BODIPY) These molecules can accurately locate the mitochondria, lysosomes and endoplasmic reticulum in the cells, and release the mitochondrial uncoupling agent, purinomycin and other drugs This achievement was published in German Applied Chemistry (DOI: 10.1002 / anie 201900850) under the title of "organelles targeted BODIPY photocases: visible light mediated sub cellular photoelease" Fig 1 The synthesis and photochemical properties of photorecage molecules (5-8, 10) (photo source: angel Chem Int ed.) the photoreactivity of BODIPY type photorecage molecules is closely related to its structure Therefore, the author first explored the influence of different structures and substituent modifications on the molecular photochemical properties On the basis of BODIPY containing p-nitroaniline (PNA), the authors synthesized different structure and functional group substituted BODIPY photocage molecules (Fig 1.5-8, 10), and studied their stability (Fig 1) The results show that these molecules are very stable under the condition of no light, and PNA will be released under the condition of visible light, which shows that the modification method adopted by the author does not change the characteristics of BODIPY as a cage molecule Fig 2 Synthesis, photochemical properties and cell co localization effect of molecular probes 11-13 (picture source: angel Chem Int ed.) after confirming that this form of modification will not affect the photochemical properties of BODIPY, the author, based on 4, obtained probes with endoplasmic reticulum localization effect through amidation reaction 11, probes with enzyme localization effect 12 And probe 13 of mitochondrial localization effect, and studied their photochemical properties and cell localization effect (Fig 2) The results showed that all the three probes could be enriched into specific organelles after entering the cells, and their PNA release characteristics were also preserved Fig 3 Synthesis and cell test of photorecage molecules 14 and 16 (image source: angel Chem Int ed.) Finally, the author tested the applicability of these photorecage molecules in practical operation with mitochondrial positioning probe as a model 2,4-dinitrophenol is a commonly used mitochondrial uncoupling agent, which can effectively reduce the mitochondrial membrane potential, while the commercial mitochondrial positioning dye rhodamine 123 (rho123) will enter the cytoplasm when the mitochondrial membrane potential is reduced Based on this characteristic, the authors studied the regulatory properties of BODIPY type cage molecule 16 with dinitrobenzene group by fluorescence microscope (Fig 3) The results showed that this kind of light cage molecule can effectively carry out light reactions at specific positions in living cells Highlight of the paper: the author reported a modification method that does not affect the characteristics of photodiode of BODIPY, and obtained a variety of photodiode molecules that can locate subcellular organelles Full text author: dnyaneshwar kand, Lorena Pizarro, INBAR angel, ADI Avni, dinorah Friedmann morvinski, and Roy weinein.
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