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The most controversial side effect of CRISPR gene editing technology, the off-target effect, has a solution.
The team involved in CRISPR-Cas9 technology, Jennifer Dudner, has confirmed that the anti-CRISPR protein can reduce the off-target effect caused by CRISPR by a quarter, and that one of the proteins, called AcrllA4, can even reduce the rate of off-target effects fourfold, with no effect on gene editing of the target site throughout the process, physicists reported Tuesday.
Dudner's lab at the University of California, Berkeley, worked with Jacob Cohen of the Institute for Innovative Genomics to study the antiCRISPR protein discovered last year and published a paper in the latest issue of the journal Science Advances.
they found that in modifying genetic variants associated with sickle cell disease, adding the AcrllA4 protein was effective in reducing off-target effects when CRISPR performed gene editing hours later.
researchers explain: "The anti-CRISPR protein simulates DNA and blocks Cas9's ability to cut genes.
off-target effect can bring unexpected mutations, but our research has shown that the off-target effect is completely controllable and not as severe as people think.
2016, UC San Francisco scientist Joseph Bundy-Denumi's team found four proteins in Liszt bacteria that block CRISPR-Cas9 activity, including AcrllA4.
another team found three other proteins from one type of meningitis bacteria.
Bondi-Denumi predicted at the time that the proteins would become a standard part of CRISPR gene therapy, allowing CRISPR-Cas9 to "turn off its activity" after a period of gene editing, preventing arbitrary shearing at unnecessary sites, resulting in off-target effects.
Kon believes that if the electric gate can only be opened and not turned off, it will be very scary, so turning off Cas9 activity is as important as turning it on, for CRISPR final treatment applications, precise regulation of Cas9 at what time, where gene editing, anti-CRISPR protein will play a vital role.
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