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    Home > Biochemistry News > Biotechnology News > Antisense RNA fine-tunes gene expression of the type II MazEF toxin-antitoxin system

    Antisense RNA fine-tunes gene expression of the type II MazEF toxin-antitoxin system

    • Last Update: 2022-01-24
    • Source: Internet
    • Author: User
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    Summary

    Despite the ubiquity of antisense RNAs, there are few functional features of antisense RNAs, which are considered by some to be transcriptional noise
    .

    Here, we report an antisense RNA (asRNA), aMEF (antisense RNA)Mazef), which plays a dual regulatory role in the type II toxin antitoxin (TA) systemMazef
    .


    Unlike the type I TA system and many other regulatory asRNAs, aMEF stimulates
    Mazefrather than inhibition and degradation


    Next-generation RNA sequencing of many organisms has shown that transcription is widespread across the genome, termed ubiquitous transcription, and is not attached to annotated gene boundaries


    However, the advent of genome sequencing demonstrates the ubiquity of genome sequencing (1 – 3) in bacteria, the most prevalent transcription occurs at (within genes) the antisense or antisense of annotated genes


    15) Type I toxin-antitoxin systems utilize asRNAs as antitoxins to regulate the expression of their cognate mRNA toxins


    MazefReverse genetic methods using targeted deletions are not feasible without disturbing overlapping coding sequences
    .
    Previously, most laboratories investigated asRNA-dependent gene regulation by overexpressing asRNAtransHowever, asRNAs are transcribed inCIScorresponding to their sense mRNAs
    .
    overexpression of asRNAtransno analog inputCISThe expression of asRNA may not reveal the regulatory effect of asRNA on mRNA
    .
    In fact, even a brief overexpressiontrans- Proxy sRNAs have many caveats (twenty one) In addition, high production of sRNAs may result in gene expression artifacts that compete with other sRNAs for binding to RNA chaperones, affecting the availability of RNA chaperones within the cell (22–26) Here, we utilize a technique for endogenous knockout of asRNA to analyze the function of asRNA (27) We identified the non-canonical aMEF promoter and made precise mutations on the chromosome to reduce the transcription level of asRNA without disrupting the open reading frame (ORF) of MazF
    .
    In addition, we also found that the aMEF promoter isE.
    Escherichia coliAlthough the sequence conservation is relatively low
    .
    These results suggest that the level of sequence conservation does not always accurately predict the level of functional conservation
    .
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