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Application Note: Ultrafiltration Solutions for Enrichment and Desalination

 Last Update: 2022-08-30
 Source: Internet
 Author: User

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pcr assay

membrane proteins

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Ultrafiltration of the test sample and buffer exchange with ultrapure water can quickly achieve desalination and also concentrate the sample.

Vivaspin 500Vivacon 500

Low sample volume? Worry about recycling rate

Vivaspin 500

I use a PES membrane with an ultra-fast flow rate, which has a fast capacity for protection, so I only have a single membrane, and the non-specific adsorption is reduced by half
.

Vivacon 500

I used a very low adsorption Hydrosart membrane, and a transverse membrane with a very small membrane area to minimize non-specific adsorption
.

How can efficiency be further improved?

Vivaspin 500

Let me tell you quietly, because I only have a single mask, the bottom space is large.
After centrifugation, the suction tip is inserted into the bottom to be completely recovered, and there is no need to reverse the centrifugation
.

How to obtain the most consistent concentration factor between parallel samples ?

Vivacon 500

I am a flat film and try to keep the centrifugal force as consistent as possible;

Vivaspin 500
is a tangential flow monolithic membrane.
In addition to centrifugal force, we should also pay attention to keeping the scale face up for each little brother.
If you use other double-sided membrane products on the market, try to make the two membranes face sideways.
, rather than up and down
.

How to prevent the protective agent on the ultrafiltration tube from
interfering with the test results?

Our ultrafiltration membranes have traces of glycerol and sodium azide to avoid membrane drying
.
Usually these protectants do not interfere with the assay
.
However, because the subsequent detection is different, if there is interference, the ultrafiltration tube can be pre-rinsed with buffer and purified water
.
If it still affects the detection, pre-rinse with 0.
1N HCl to remove interfering peaks
.
Vivaspin 500 has PCR grade products, which can be used if it is necessary to avoid nucleic acid contamination during nucleic acid isolation
.

^1Insulin A-chain peptide (MW 2.
5kD) and calcitonin (MW 3,593 Da) were concentrated by scientists at the University of Cambridge using a Vivaspin 500 3kD product by centrifugation at 13,000 rpm
.
Despite the small molecular weights of the two proteins, the recoveries were 98% and 99%, respectively
.

Our ultrafiltration little brother has a variety of pore sizes:

Vivaspin 500
(3kD, 5kD, 10kD, 30kD, 50kD, 100kD, 300kD, 1000kD)

Vivacon 500
(2kD, 10kD, 30kD, 50kD, 125kD)

regardless of processing virus, protein, nucleic acid samples All can show their talents
.

It is better to have a try.

Interested friends are welcome to apply for a free sample trial!

Apply for a free trial now
*The trial quota is limited, first come first served
*The final interpretation right of this event belongs to Sartorius China

- References -
1.
Alan R.
Johnson, Christine Turner
Anatomy Department, University of Cambridge, Downing Street, Cambridge, CB2 3DY.
UK
Lab filtration Application note 2001

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