Application of naica® Droplet Chip Digital PCR System in Supporting New Ideas for Detection of Single Escherichia coli

Guided reading

Despite the rapid development of health systems in various countries, infectious diseases caused by pathogenic bacteria remain one of the major threats to human health

Scientists from the Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, have developed a single bacteria detection method based on the naica ^® automatic microdroplet chip digital PCR system, which can detect single bacteria within 1.

Application highlights:

▶ The dDRCA system can rapidly and selectively detect E.

▶ Demonstrated the potential clinical applicability of the dDRCA system in the diagnosis of urinary tract infections, dDRCA was able to successfully identify 5 patients with UTI from 20 clinical urine samples in less than 1.

In this paper, the droplet microfluidic technology of the naica️ ^®

Amplification typically takes about 4 hours for quantitative E.

▲ Figure 2(a) Analysis of RCA product (RP) by agarose gel electrophoresis

The resulting fluorescent droplets were imaged and analyzed using the Naica Prism3 reader

The signal responses were also examined in the presence of three other common bacteria, including B.

▲ Figure 4(a) Fluorescence images of dDRCA reactions at different E.

The potential clinical applicability of the dDRCA system in the diagnosis of urinary tract infections (UTIs) was finally demonstrated

▲ Figure 5(a) Detection of Escherichia coli in urine samples

The system was able to successfully identify five UTI patients from 20 clinical urine samples in an analysis time of less than 1.

Original: https://doi.

naica ^® Six-Channel Digital PCR System

^{The naica ®} six-channel digital PCR system from Stilla Technologies, a French company , is derived from the Crystal microdroplet chip digital PCR technology.