Application of sCMOS camera high-speed imaging performance in the biological field (II)

Third, to capture the rapid changes of fluorescent signals Many physiological and bio-chemical processes are accompanied by rapid changes in fluorescent signals.
can sometimes be discerned by the naked eye for these rapidly changing signals, which can be captured well with Flash 4.0's high-speed imaging.
In the image above, the naked eye can't see the fluorescent signal changes of neuron cells due to changes in membrane position, but after time analysis of the grayscale of the image under high-speed imaging, you can see the rapid changes in fluorescence within the 1ms time scale.
other changes that can be observed by the naked eye, and high-speed imaging can better study the process by which their signals change over time.
such as calcium ion fluorescent dyes or fluorescent proteins (Fluo-4, GCaMP, etc.), the fluorescence intensity increases with the increase in calcium ion concentration.
, signal transducting and downstream effects record flash 4.0 at high speeds and can also record the transducting of biological signals and the downstream effects of cells below the millisecond level.
for example, after simultaneous imaging of the cell calcium ion signal and the bright field difference of cell contraction lysus, the grayscale change of the quantitative analysis image can obtain the time information of the two events.
two time curves we can see that the calcium ion signal changes first, and then the cell shrinks and deforms, the time difference between the two is the calcium ion signal transduction time.
iPS induced differentiation of myocardial cell throbbing and calcium signal change application conditions: fluorescent high-speed imaging with the bright field for simultaneous imaging, the need for high sensitivity and high-speed simultaneous sCMOS camera, and with two-color splitter components, the machine needs more than 200fps, camera QE needs more than 80%.
models recommended: Flash4.0 V3/Flash4.0 LT-View Gemini V, with the application of high-end imaging methods, rapidly evolving micro-imaging technology is constantly demanding the performance of optical detectors.
in depth imaging of thick samples or ultra-resolution imaging of subcellular structures, Flash 4.0 cameras have a place in the imaging detector field for their superior performance.
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