echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry

    Assessment of Cellular Uptake and Cytotoxicity of Carbon Nanotubes Using Flow Cytometry

    • Last Update: 2020-12-29
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    The field of carbon nanotube (CNT) functionalisation is increasingly growing for the purpose of enhancing the biocompatibility of CNT for medical and biological applications. Properties of CNT such as the type of functionalisation, charge density, and the dispersibility profile are expected to modulate CNT cellular uptake and toxicity profile in vitro. The assay described here allows for rapid screening of CNT cellular uptake in vitro and assessing the acute cytotoxicity simultaneously. CNT cellular uptake is measured qualitatively by light scattering analysis without differentiating between cell binding and internalisation of the CNT by the cells. In addition, flow cytometry is used to combine light scattering analysis with flow cytometry-based Annexin V/propidium iodide assay to measure the cytotoxicity. This assay is rapid, reliable, and allows for comparative analysis between various types of CNT studied.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.