ATF-based induction chemical small molecule detection platform.

Sensitivity detection of small molecules is an important requirement in the fields of clinical diagnosis, environmental monitoring and food safety, so it is important to develop efficient chemical small molecule detection methods.
, the specific identification element is the core and basis for the realization of detection, the traditional detection method is through antibodies, enzymes, etc. as identification elements and objects to be detected, and then through the amplification of the identification signal to achieve detection.
the whole development process is often faced with long cycle, difficulty, signal output effect is not ideal and so on.
In response to the above questions, the Yang Kezing task force of the Institute of Microbiology of the Chinese Academy of Sciences, based on years of understanding of the regulatory mechanism of the primary nuclear biological transcription factor (aTF), constructed the detection platform of the inductive chemical small molecules by combining the in-body aTF of the primary nuclear organism as an identification element with the Alpha (image) signal amplification system (see figure).
aTF contains DNA binding domains and small molecule recognition domains, the binding of small molecules enables aTF to be dissoced from specific DNA sequences.
the core design of the platform is to secure the biotin-labeled aTF identification sequence DNA and aTF to the Donor microbeads and TheAcceptor microbeads, respectively.
When there are no small molecules to be detected, the Donor microbeads and TheAcceptor microbeads come close by aTF-DNA interaction, and the Donor microbeads can spread to the Acceptor microbeads by absorbing a large amount of single-line oxygen (1O2) produced by exciting light, making the Acceptor microbeads glow; When the presence of small molecules to be detected, small molecules combined with aTF to dissophuse it from DNA, so that The Donor microbeads and Acceptor microbeads separate, single-line oxygen (1O2) can not spread from the Donor microbeads to the Acceptor microbeads, so that the Acceptor microbeads can not glow.
this, small molecular concentrations are amplified by the Alpha signal for high sensitivity detection.
Yang Kezing task force used this idea to establish a certain universal testing platform, and for the clinical marker uric acid and antibiotic residual tomycin detection, respectively, developed the most sensitive detection methods.
Because of the presence of aTF in primary nuclear organisms that sense a variety of small molecules, combined with this detection platform and a large number of aTF resources, high sensitivity, new detection methods and kit development can be made for different target small molecules.
that the results have now been patented and published (Chem. Commun.cover article, click on the lower left corner to read the original text).
published this article, Li Shanshan of the Institute of Microbiology is the first author, and researcher Yang Kezhan and associate researcher Wang Weishan (a member of the Youth Promotion Association) are side-by-side correspondents.
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