Autoclave method

The principle of high-pressure steam sterilization method is that water boils at about 100 ℃ in the atmosphere, the pressure of water vapor increases, and the temperature will increase when it boils Therefore, in a closed high-pressure steam sterilizer, when the pressure indicated by the pressure gauge increases to 15 pounds (1.05 kg / cm), the temperature is the same as 121.3 ℃, and at this temperature for 20 minutes, the reproduction and spores of bacteria can be completely killed During sterilization, add enough water for once sterilization to the interlayer Fix the bottom of the iron cover on the top of the pot with a screw to prevent air leakage After heating, the steam rises along the interlayer Enter the inner cylinder from the hole When the steam enters the inner cylinder for the first time, the upper and lower exhaust pipes shall be opened After the cold air is released, the exhaust pipe shall be closed As the steam enters the closed space, the pressure rises From the pressure gauge, it can be seen that the steam also passes through the safety valve to ensure that the pressure is maintained at a certain limit, so that the pressure is maintained at 15 pounds and 20 pounds Min, then turn off the heat source and gradually reduce the pressure to zero, then open the lid of the pot Otherwise, due to the sudden pressure drop, the liquid in the glassware will rush out of the bottle mouth, causing overflow or explosion 1 Connect the power supply 2 Check the water level of the cooling bottle at the bottom right of the autoclave to ensure that the water level is between Zui high water level and Zui low water level Note: do not bend the steam drain pipe and block the vent hole, otherwise it is not easy to open the high-pressure boiler door after sterilization 3 Add distilled water into the pot, and the horizontal surface shall exceed the stainless steel plate at the bottom of the sterilization pot 4 Turn on the upper left power switch The initial setting value of Zui is 120 ℃ / 20 minutes 5 Sterilization of articles: put the articles to be sterilized into the stainless steel basket Note: do not put the articles into the pot directly, otherwise the exhaust port and safety valve will be blocked and the high-pressure pot will be out of control, which will lead to danger 6 Close the cover of the autoclave Hold the handle of the high-pressure pot cover with both hands and tighten it clockwise for about 2 turns When you feel mechanical resistance, rotate it for another 1 / 4 turn to ensure it is closed tightly 7 Close the exhaust valve Turn the handle clockwise until it stops, and further tighten it to ensure safety Note: the handle of exhaust is not closed tightly, the pressure does not rise after sterilization, and an alarm will be given 8 Set the required sterilization temperature and time 9 Start sterilization Press the start key, the sterilization display flashes to show the start of sterilization operation When the temperature reaches the preset temperature, the sterilization display stops flashing and remains bright 10 End of sterilization After sterilization, the machine will automatically sound the prompt and open the exhaust valve to speed up the exhaust speed Note: if the pot is filled with test tube blocked by cotton plug, do not open the vent valve too much, otherwise the sudden decrease of pressure will make the cotton plug pop out or the glassware break 11 Sterilization completed After the steam in the high-pressure boiler is discharged, the pressure pointer points to 0, and the temperature is below 90 ℃, open the boiler cover to dissipate heat 12 Take out the sterilized articles and close the power supply and the pot cover Make use records