Automation of MLST using third-generation liquid-handling technology
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Last Update: 2021-03-02
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Source: Internet
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Author: User
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The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant strains to be monitored, and for the impact of new vaccines to be assessed. Our laboratory performs MLST on
Neisseria meningitidis, Streptococcus pneumonaie, Haemophilus influenzae
, and
Staphylococcus aureus
. We have developed high-throughput automated methods to allow MLST to be performed in a time scale useful in a clinical setting. Here we describe the automation of MLST on a third-generation liquid-handling robot.
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