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    Home > Food News > Food Articles > Baifan team revealed a new mechanism for E. coli whip hair growth

    Baifan team revealed a new mechanism for E. coli whip hair growth

    • Last Update: 2021-03-10
    • Source: Internet
    • Author: User
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    May 14, 2018, Bai Fan, a team of researchers at the Center for Biodynamical Optical Imaging at Peking University's School of Life Sciences, published a research paper in the journal Nature Communications entitled "Frequents in Escherichia coli flagella elongation revealed by single cell cell-real fluorescence." In this paper, the researchers realized for the first time real-time dynamic fluorescence imaging of the growth of E. coli's weekly whiplash, and delved into the assembly process of whiplash. The study found that E. coli whiplash of the same length had a large difference in growth rates, which were due to stagnation in the assembly of whiplash. Through further experiments and mathematical models, the paper reveals that insufficient supply of whiplash sub-base proteins in E. coli cytokines can cause stagnation in whiplash growth, and this study is important for understanding the extracellal transport and assembly of bacterial proteins.
    E. coli relies on the rotation of whiplash for swimming, but the growth process of whiplash has not yet been studied clearly. In the past, data from group measurements suggest that the growth rate of E. coli whiplash remains constant. In recent years, the progress of fluorescence imaging technology has enriched the research method of whiplash growth mode. In this paper, researchers inserted four cysteine labels into E. coli's whiplash subkey-FliC protein and tagged whiplash with FlAsH/ReAsH fluorescent dyes, combining real-time fluorescence microimaging technology with structural light illumination ultra-resolution imaging techniques, to explore the relationship between E. coli whipping patterns and the growth rate of multiple whiplashes on the same bacteria.E. E. coli whiplash fluorescence markers and ultra-resolution microscopic imaging
    The researchers first used two-color/tricolor fluorescence markers and combined with structural lighting ultra-distinguished micro-imaging technology to count the growth rate of E. coli whiplash, and found that the growth rate of E. coli whiplash at the group level was negatively related to the length of whiplash, and the growth rate of different whiplashes at the same length was significantly different. At the single whiplash level, the researchers used real-time fluorescence microimaging technology to track the continuous growth of a single whiplash, and counted the results of several sets of experiments, and found that E. coli whiplash in the growth process is generally stagnant growth phenomenon; By over-expression of whiplash sub-protein FliC in cells, the researchers found that the rate of stagnation in the growth of E. coli whiplash decreased significantly, revealing that the shortage of FliC supply in cells is an important reason for the stagnation of E. coli whiplash growth. Finally, the researchers further expounded and analyzed the effects of FliC concentration changes on whiplash growth by establishing a mathematical model.E. coli whiplash growth rate measurement
    Bai Fan researcher and Professor Luo Jianrong are co-authors of the paper. This study is supported by the National Natural Science Foundation of China and the Human Frontier Science Program (HFSP). (Source: Science Network)
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