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Biochemical Analyzer Basic measurement method and principle (i): The end-point method is a quantitative analysis of the substance according to the absorption spectral characteristics of the reaction product when the reaction reaches equilibrium and its absorbance size For general chemical reactions, the reaction is complete (or positive or reverse dynamic equilibrium), and when the reaction product is stable, the reaction endpoint is the reaction Anti-primary antibody reaction, is the antigen and antibody full response, the formation of zui large and stable immune complex as the end point On the reaction time process curve is a segment parallel to the x-axis
(ii): Continuous monitoring method is also known as rate method That is, the method of continuous monitoring of the reaction process, quantitative analysis according to the rate of product generation or substrate consumption On the reaction time process curve, the reaction is constant-speed segment (slope remains constant), and is often used for enzyme activity linear reaction period determination
(iii): Blank correction In the spectrophotometric method, blank solution is often used to regulate the absorption of the instrument at zero points, or to counteract certain assay interference factors In biochemical analyzer determination, in addition to the use of double or multi-wavelength, two-point method and other elimination of background interference, it is often necessary to use a special blank determination, in order to determine the effect shorter from the sample measurement absorption Proper selection of blank correction plays an important role in improving accuracy
1 Reagent blank swashes are generally in the method type and calibration mode, that is, divided into two categories with or without reagent blanks
Reagent blanks are measured individually or in conjunction with calibration, and are pre-selected to be loaded with deionized water sample cups or reagent blank scaffolds The absorption of light absorption at each measuring point of calibration or patient sample shall be deducted from the reagent blank absorbance or blank rate value of the corresponding measurement point No reagent blank method, more directly to the reaction cup water blank as the determination of the benchmark value
In many instruments, reagent blank determination is similar to calibration assays, not in real time when a patient sample is measured Therefore, pay attention to its measurement frequency, to avoid the change of reagent batch number or quality caused by the change in reagent blank calculation error
2 Sample blank is mainly to eliminate the confusion or color interference of the sample itself The blank channel method is often used to determine the correction result - color-resonating reaction channel result - blank channel result Most instruments have to occupy the measurement channel separately, the analysis speed is halved, but the accuracy of the de-interference should be higher than the two-point end method.