Beijing Academy of Agriculture and Forestry Sciences has made important progress in the study of the whole genome structural variation of cauliflower and cabbage and the molecular mechanism of important organ formation

Original title: Beijing Academy of Agriculture and Forestry Sciences has made important progress in the study of the structural variation of the whole genome of cauliflower and cabbage and the molecular mechanism of formation of important organs

Recently, the Beijing Academy of Agriculture Liu Fan, a researcher team, jointly Jiangsu Academy of Agricultural Sciences and American scientists and the Netherlands, in biological important areas of science journal "BMC Biology" Online published an article entitled "Genome sequencing sheds light on the contribution of structural variants to Brassica oleracea diversification " of the research paper (Guo et Al.

Cabbage (Brassica oleracea) is a diploid basic species of Brassica of the cruciferous family.

In this study, the researchers used the third-generation single-molecule real-time sequencing (PacBio SMRT) combined with optical physical mapping (BioNano) and chromatin conformation (Hi-C) technologies to successfully assemble cauliflower (Korso) and bovine heart-shaped globules Cabbage (OX-heart) chromosome-level high-quality reference genome (N50 is 4.

Based on the assembly and annotation of high-quality reference genomes, and comprehensively using multiple methods of comparative analysis, a total of about 120,000 structural variations were obtained between the Korso and OX-heart genomes

Finally, the researchers combined the genes affected by Selected-SVs and the transcriptome data of cauliflower curd development to identify a batch of candidate genes related to curd development, and initially revealed that FLC, WUS, CAL, SVP, ARL2 and other genes are in the curd.

Genome structural variation can cause large-scale changes in cis-regulatory regions, which are more likely to change gene expression levels and affect phenotypes, and play a very important role in plant evolution and the formation of agricultural traits

Associate researcher Guo Ning from the Vegetable Center of our institute, researcher Wang Shenyun from the Vegetable Institute of Jiangsu Academy of Agricultural Sciences, researcher Gao Lei from Wuhan Botanical Garden of Chinese Academy of Sciences, and Liu Yongming from the Future Group are the co-first authors of this paper; Guusje Bo, Wageningen University, NetherlandsProfessor nnema, researcher Li Jianbin from the Vegetable Institute of Jiangsu Academy of Agricultural Sciences, Professor Fei Zhangjun from the Boy Thompson Institute of the United States, and researcher Liu Fan from the Vegetable Center are the co-corresponding authors.

In recent years, this research group of the Vegetable Research Center has focused on cauliflower, and has successively carried out double haploid, somatic distant hybridization, gene editing and other cell and genetic engineering breeding technologies and germplasm innovation research.

(This article is reprinted from "Food Science Network", and the article comes from Beijing Academy of Agriculture and Forestry Sciences