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Bermudagrass (Cynodon spp.)

 Last Update: 2021-01-13
 Source: Internet
 Author: User

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Bermudagrass is an important warm-season forage and turf species widely grown in the southern United States. This chapter describes a rapid and efficient protocol that allows for the generation of a large number of transgenic bermudagrass plants, bypassing the callus formation phase. Stolon nodes are infected and co-cultivated with
Agrobacterium tumefaciens
harboring pCAMBIA binary vectors. Hygromycin phosphotransferase gene (
hph
) is used as the selectable marker and hygromycin is used as the selection agent. Green shoots are directly produced from infected stolon nodes 4 to 5 wk after hygromycin selection. Without callus formation and with minimum tissue culture, this procedure allowed us to obtain well-rooted transgenic plantlets in only 7 wk and greenhouse-grown plants in only 9 wk.

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