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    Home > Bian Liming team, Chinese University of Hong Kong: heterodimer magnetic nanoswitch for remote regulation of stem cell adhesion and differentiation

    Bian Liming team, Chinese University of Hong Kong: heterodimer magnetic nanoswitch for remote regulation of stem cell adhesion and differentiation

    • Last Update: 2018-05-22
    • Source: Internet
    • Author: User
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    In order to regulate the cell function in vivo, the introduction requires a remote, noninvasive and reversible nano level platform to realize the nano scale presentation of bioactive ligands Recently, Professor Bian Liming of the Chinese University of Hong Kong put forward an innovative method: the magnetic controlled nano heterodimer switch was used to regulate the dynamic binding of RGD peptide to cell membrane surface receptor (DOI: 10.1021 / JACS 8b03001) Brief introduction of Professor Bian Liming's research group Professor Bian Liming's research group has been devoted to the research of biomaterials and tissue engineering for a long time Based on natural polymers such as hyaluronic acid and gelatin, a series of functional hydrogels with good mechanical properties and biocompatibility have been developed, and the bioactivity of hydrogels and their important applications in regenerative medicine have also been concerned At the same time, based on a variety of nano materials, the research team developed a new system for gene / drug delivery and molecular imaging, and made many new progress in the fields of regulating stem cell differentiation and studying cell-material interaction In recent years, Professor Bian Liming has been responsible for a number of scientific research projects as project leader, including the fund of the Hong Kong Research Grants Council, the Hong Kong health medical research fund, the fund of the Hong Kong Innovation and Technology Department, and the National Natural Science Foundation At present, the research group has seven doctoral students, one research assistant and four postdoctoral students During the five years since the establishment of the research group, the members of the research group have published more than 50 SCI papers in relevant fields, including articles published in J am Chem SOC., PNAs, adv funct Material., ACS Nano, nano letters, biomaterials, NPG Asia materials, small, macrolecules and other journals with Professor Bian Liming as the corresponding author Prof Bian Liming: Prof Bian Liming received his Ph.D in biomedical engineering from Columbia University in 2009, and engaged in postdoctoral research from the University of Pennsylvania from 2009 to 2012 He is currently an assistant professor of Biomedical Engineering at the Chinese University of Hong Kong Cutting edge scientific research achievements: the heterodimer magnetic nano switch developed by liming team of heterodimer remote regulation of stem cell adhesion and differentiation consists of magnetic nano cage (MNC), polyethylene glycol (PEG), RGD and gold nano particle (AuNP) on glass substrate The displacement caused by MNC induced by magnetic field allows reversible entrapment and unsealing of RGD polypeptide on AuNP, so it can physically regulate the binding of RGD to integrins of cells, thus regulating the adhesion of human stem cells to matrix in vitro and in vivo, and controlling stem cell differentiation and regulating the mechanical transmission of information in cells The novel design of using heterodimer magnetic nanoswitch to unseal RGD reversibly provides an effective remote controller for regulating cell line in vivo In recent years, the use of remote operation to regulate the interaction between cells and materials in vivo and in vitro (such as the interaction between integrins and ligands) is mainly controlled by photochemistry (near infrared, ultraviolet, etc.) However, light penetration is limited and may cause potential damage to somatic cells Therefore, it is an ideal strategy to use noninvasive magnetic control with high penetration and relatively harmless to regulate the interaction between cells and materials Last year, Bian Liming's team reported that RGD loaded superparamagnetic nanoparticles (MNP) were modified on the surface of the material through polyethylene glycol chains, thus developing an innovative nano platform for interaction between magnetic controlled stem cells and macrophage integrins and ligands In this study, the team designed a more innovative and simple magnetically controllable heterodimer nano cage to further demonstrate the application and advantages of the magnetically controlled nano platform Fig 1 Reversible blocking and unlocking of heterodimer magnetic nanocage switch to regulate stem cell adhesion and differentiation in vivo (source: J am Chem SOC.) Fig 2 In situ imaging and dynamic image of the magnetic nano cage switch (source: J am Chem SOC.) the author observed the heterodimer magnetic nano cage with a transmission electron microscope, and its shape was a superparamagnetic nano particle and a gold nano meter The results show that MNC - (aunp-rgd) heterodimer coupling substrate is mainly composed of gold and iron The imaging results of AFM show that the heterodimer can generate reversible switch through magnetic field, and the blue dotted line (Figure 2a) is the center of the nonmagnetic reference material AuNPs Under the strong magnetic field, MNC is pulled away, and after the exposure of aunp-rgd, it is easy to interact with integrins and adhesion ligands In the absence of magnetic attraction, the MNC cage is naturally closed, and integrins cannot interact with aunp-rgd Fig 3 The heterodimeric magnetic nanocage switch can reversibly regulate the adhesion and differentiation of stem cells at different time points (source: j.am Chem SOC.) when the interaction between integrin and adhesion ligand and aunp-rgd reaches maturity, the adhesion site will form adhesion spots, and the important proteins forming this structure are vinculin and mechanosensitive transcription factor yap When the heterodimeric magnetic cage is closed, the cells can not form adhesion spots in the first 24 hours; when the heterodimeric magnetic cage is opened, the cells can form adhesion spots in the first 24 hours At the same time, the cells can react to the heterodimeric magnetic nanocapsules opened and closed at different times For example, when the cage is opened at the first 12 hours, the cells can form adhesion spots; but when the cage is closed at the last 12 hours, it cannot, and vice versa Since the adhesion spots and Yap play an important role in the osteogenic differentiation of stem cells, opening the heterodimeric magnetic nano cage can benefit the early proteins ALP and Runx2 expressed in the osteogenic differentiation, while closing it is not conducive to the osteogenic differentiation Similarly, switching at different times can be beneficial or detrimental to its differentiation Figure 4 The remote control of the heterodimer magnetic nano cage switch changed the adhesion of stem cells in vivo and the induction of mechanical mechanics (source: j.am Chem SOC.) Finally, the team placed the platform in mice for relevant experiments, the results were the same as those in vitro, proving that the heterodimer magnetic nano cage switch can be applied in vivo Conclusion: the team of Bian Liming from the Chinese University of Hong Kong has developed MNC - (aunp-rgd) heterodimer nano cage This study provides evidence for the first time that reversible ligands can be decoiled by magnetic force, which enables nanoswitch to control stem cell adhesion in vitro and in vivo Reversible magnetic nanoswitch allows time regulated RGD release Therefore, the magnetic nanoswitch can be used for time regulation of various cell functions in vivo This physical, non-invasive, non-contact, reversible nanoswitch may improve the performance of the material implant and the effect of stem cell regeneration therapy The results of this study were recently published in J am Chem SOC The postdoctoral work of the research group of Bian Liming of the Chinese University of Hong Kong and the postdoctoral work of the research group of Professor vinayak dravid of the Northwestern University of the United States, he Joon Jung, is the co-author, and Professor Bian Liming is the corresponding author Nowadays, people and scientific research have been paid more and more attention in the economic life China has ushered in the "node of science and technology explosion" Behind the progress of science and technology is the work of countless scientists In the field of chemistry, in the context of the pursuit of innovation driven, international cooperation has been strengthened, the influence of Returned Scholars in the field of R & D has become increasingly prominent, and many excellent research groups have emerged in China For this reason, CBG information adopts the 1 + X reporting mechanism CBG information, chembeango app, chembeango official microblog, CBG wechat subscription number and other platforms jointly launch the column of "people and scientific research", approach the domestic representative research group, pay attention to their research, listen to their stories, record their demeanor, and explore their scientific research spirit.
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