Bio-clock protein can prevent liver-sourced diarrhea in piglets

, the Journal of Livestock and Biotechnology published online the latest results of the team of Cai Demin, a professor at Yangzhou University's School of Animal Science and Technology. This paper expounds the regulation of the bio-clock protein ROR on the synthesis of bile acid.
Cai Demin, about 25% of the world's grain is contaminated with a variety of molds each year, and China is the hardest hit by mycotoxins. Mycotoxin can enter the pig body through feed, resulting in reduced feeding, growth retardation, poor feed remuneration, decreased immune function and other issues, serious mycotoxin poisoning will cause sow abortion, intestinal and kidney bleeding, liver and bile swelling damage cancer, and even cause liver damage, diarrhea and death of piglets.
present, the commonly used way of handling mycotoxins in the aquaculture industry is difficult to remove moldy feed from the tank, and there is no cure for sick pigs. So, can we start from the body anti-toxic to prevent piglet liver-sourced diarrhea?
the study, when bile is insufficient, milk fat cannot be effectively absorbed in the intestines, remaining in the intestines, causing the cells in the intestinal wall to secrete too much water, causing diarrhea and rapid dehydration. At present, it is believed that the nuclear subject is the "switch" to control bile synthesis, and the three nuclear subjects of ROR, LXR and REV-ERB regulate bile synthesis by the influence of the synthetic bile acid key enzymes CYP8B1 and CYP7A1.
"We guess that under the action of mycotoxin, the piglet liver nucleus subject ROR is deacetylized on the genes associated with bile acid synthesis, the binding degree of ROR gamma to the target gene decreases, and the transcription cofactor of ROR's recruitment decreases, resulting in blocked transcription, decreased expression and reduced cholic acid synthesis. Therefore, regulating bile acid metabolism has become a target for the prevention and treatment of piglet liver-sourced diarrhea. Zhang Jiaxin, the paper's first author, said.
ROR is an important biological rhythm-regulating protein, after verifying the relationship between ROR gamma and bile acid metabolism, the study found that in the liver of diarrhea piglets, the path of cholic acid synthesis is blocked, and the expression of ROR gamma and its genes is significantly reduced. After the expression of ROR gamma, the content of bile acid and cholesterol and the expression of their synthetic-related genes increased.
same time, the team successfully detected the binding point of ROR gamma in the liver of pigs through chromosomal immunococipitation techniques. By comparison, it was found that ROR gamma in the diarrhea group had lower binding ability in the startup subregion.
team further constructed a model of piglet liver organs in-body for bio-clock remodeling, and found that both biological rhythms and the expression rhythms of genes associated with bile acid synthesis were disrupted. Among the 20 lipid-related small molecule compounds screened, the team found that the decline in bile acid content and the expression levels of genes associated with cholic acid synthesis caused by the biological clock disorder was restored only under the action of ROR's small molecule astrogen SR0987 and streptool.
to further verify the effect of ROR astitid streptol on piglet liver-based diarrhea, the study divided piglets into mycotoxin group, mycotoxin-chain sterol group and control group. The experimental results showed that in the mycotoxin group, the diarrhea rate of piglets was higher and the mortality rate was up to 50%, while in the group with streptomol, the diarrhea rate of piglets was significantly reduced and the survival rate was greatly improved.
The study suggests that, on the one hand, streptomol is a natural product widely distributed in algae plants and can be used as an algae extract to develop functional feed to prevent the occurrence of liver-based diarrhea in piglets. On the other hand, streptosterols can be used to develop new veterinary drugs that precisely target the treatment of liver-sourced diarrhea. These two innovative applications in "prevention" and "cure" can greatly reduce the impact of liver-sourced diarrhea on the pig industry, reduce piglet mortality, stabilize pig production, and play an important role in restoring pig storage. (Source: Li Chen Yuxuan, China Science Journal)
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